Neuroscience and Inflammation Unit, Department of Physiology, College of Health Sciences, University of Ilorin, Ilorin, Kwara, Nigeria.
Sci Rep. 2022 Feb 24;12(1):3196. doi: 10.1038/s41598-022-06554-4.
Neuropathic pain (NP) is an abnormality resulting from lesion or damage to parts of the somatosensory nervous system. It is linked to defective quality of life and often poorly managed. Due to the limited number of approved drugs, limited efficacy and side effects associated with the approved drugs, drugs or drug combinations with great efficacy and very minimal or no side effects will be of great advantage in managing NP. This study aimed at investigating the synergistic antinociceptive effects of the combination of glucosamine sulphate (GS) (240 mg/kg) and chondroitin sulphate (CS) (900 mg/kg) in chronic constriction injury (CCI)-induced neuropathy in rats. Forty-two Wistar rats were randomly distributed into seven groups (n = 6). Sciatic nerve was ligated with four loose ligatures to induce NP. Effects of drugs were examined on stimulus and non-stimulus evoked potentials, expression of dorsal root ganglia (DRG) pain modulators and structural architecture of DRG. Oral administration of GS and CS for 21 days reduced hyperalgesia, allodynia, sciatic nerve functional aberration and DRG pain modulators. Histopathology and immunohistochemistry revealed restoration of structural integrity of DRG. Our result showed that the combination of GS and CS produced antinociceptive effects by attenuating hyperalgesia, allodynia and downregulation of NP mediators. GS and CS additionally produced synergistic analgesic effect over its individual components.
神经病理性疼痛（NP）是一种由躯体感觉神经系统的损伤或病变引起的异常。它与生活质量下降有关，且往往难以治疗。由于批准的药物数量有限，批准药物的疗效有限且存在副作用，因此具有高效、副作用极小或无副作用的药物或药物组合将在 NP 的管理中具有巨大优势。本研究旨在研究硫酸氨基葡萄糖（GS）（240mg/kg）和硫酸软骨素（CS）（900mg/kg）联合应用对慢性缩窄性损伤（CCI）诱导的大鼠神经病理性疼痛的协同抗伤害作用。42 只 Wistar 大鼠随机分为 7 组（n=6）。用 4 个松结扎坐骨神经以诱导 NP。检测药物对刺激和非刺激诱发的动作电位、背根神经节（DRG）疼痛调节剂的表达和 DRG 结构的影响。GS 和 CS 口服给药 21 天可减轻痛觉过敏、触诱发痛、坐骨神经功能障碍和 DRG 疼痛调节剂。组织病理学和免疫组织化学显示 DRG 的结构完整性得到恢复。我们的结果表明，GS 和 CS 的联合应用通过减轻痛觉过敏、触诱发痛和下调 NP 介质产生了镇痛作用。GS 和 CS 还产生了比其单独成分更协同的镇痛作用。
