Li Jie, Zhang Feng-Quan, Du Zhen-Ning, Cai Teng, Cai Peng-Shan, Fan Lei
Department of Emergency Surgery, Henan Provincial People's Hospital, Zhengzhou, 450003, China.
School of Public Health, Xinxiang Medical University, Xinxiang, 453003, China.
J Huazhong Univ Sci Technolog Med Sci. 2015 Jun;35(3):374-377. doi: 10.1007/s11596-015-1440-3. Epub 2015 Jun 14.
Heme oxygenase-1 (HO-1) plays important roles in anti-oxidant, anti-inflammatory and immunoregulative activities. The aim of this study was to observe if HO-1 transfection could inhibit the damage of osteoblasts induced by ethanol. HO-1 was transfected into osteoblasts via constructed plasmid. After exposure to ethanol for 24 h, cytoactivity and apoptosis of osteoblasts were measured by MTT assay and flow cytometry, respectively. Furthermore, the oxidative stress and inflammatory factors in osteoblasts were measured. Compared to positive control group, the cytoactivity of transfected osteoblasts was significantly increased, and the apoptosis rate was significantly decreased (P<0.05). At the same time, the levels of reactive oxygen species (ROS), methane dicarboxylic aldehyde (MDA), tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1) were significantly decreased (P<0.05), and superoxide dismutase (SOD) level was increased (P<0.05) in the transfected osteoblasts as compared with positive controls. These results suggest that HO-1 plays a protective role in osteoblasts, and HO-1 transfection can effectively inhibit bone damage induced by ethanol.
血红素加氧酶-1（HO-1）在抗氧化、抗炎和免疫调节活动中发挥着重要作用。本研究的目的是观察HO-1转染是否能抑制乙醇诱导的成骨细胞损伤。通过构建质粒将HO-1转染到成骨细胞中。在乙醇处理24小时后，分别通过MTT法和流式细胞术检测成骨细胞的细胞活性和凋亡情况。此外，还检测了成骨细胞中的氧化应激和炎症因子。与阳性对照组相比，转染后的成骨细胞细胞活性显著增加，凋亡率显著降低（P<0.05）。同时，与阳性对照组相比，转染后的成骨细胞中活性氧（ROS）、丙二醛（MDA）、肿瘤坏死因子-α（TNF-α）和白细胞介素-1（IL-1）水平显著降低（P<0.05），超氧化物歧化酶（SOD）水平升高（P<0.05）。这些结果表明，HO-1在成骨细胞中起保护作用，HO-1转染能有效抑制乙醇诱导的骨损伤。
