Zhou Ping, Xia Jie, Zhou Yong-Jie, Wan Jun, Li Li, Bao Ji, Shi Yu-Jun, Bu Hong
Ping Zhou, Jie Xia, Yong-Jie Zhou, Jun Wan, Li Li, Ji Bao, Yu-Jun Shi, Hong Bu, Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
World J Gastroenterol. 2015 Jun 7;21(21):6665-74. doi: 10.3748/wjg.v21.i21.6665.
To investigate whether the proportions of acetyl-histone-positive hepatocytes could be used as markers of deteriorating liver function.
In total, 611 cirrhotic cases from 3701 patients who were diagnosed during the past 15 years were screened, and 152 follow-up cases were selected. Paraffin tissue microarray was prepared for immunohistochemistry to examine acetyl-histone expression. The proportions of positive hepatocytes were recorded, and their correlations to clinical and laboratory indicators were analyzed statistically.
The proportions of H2AK5ac(+), H3K9/K14ac(+) and H3K27ac(+) hepatocytes gradually increased with deteriorating liver function and with increasing levels of serum markers of liver injury. In the follow-up cases, patients with > 70% H2AK5ac(+), H3K9/K14ac(+) or H3K27ac(+) hepatocytes had statistically lower survival rates (P < 0.05). Furthermore, > 70% H2AK5ac(+) or H3K27ac(+) hepatocytes were strong independent predictors of overall survival (P < 0.05).
The proportions of acetyl-histone-positive hepatocytes are closely associated with the liver function and prognosis of cirrhotic patients.
研究乙酰化组蛋白阳性肝细胞的比例是否可作为肝功能恶化的标志物。
对过去15年中确诊的3701例患者中的611例肝硬化病例进行筛选，并选取152例进行随访。制备石蜡组织芯片进行免疫组化以检测乙酰化组蛋白的表达。记录阳性肝细胞的比例，并对其与临床和实验室指标的相关性进行统计学分析。
H2AK5ac(+)、H3K9/K14ac(+)和H3K27ac(+)肝细胞的比例随着肝功能恶化和肝损伤血清标志物水平升高而逐渐增加。在随访病例中，H2AK5ac(+)、H3K9/K14ac(+)或H3K27ac(+)肝细胞比例>70%的患者生存率在统计学上较低（P<0.05）。此外，H2AK5ac(+)或H3K27ac(+)肝细胞比例>70%是总生存的强有力独立预测因素（P<0.05）。
乙酰化组蛋白阳性肝细胞的比例与肝硬化患者的肝功能及预后密切相关。
