Oyamatsu T, Shimizu N, Takeuchi K, Yamamoto M, Kawai Y, Watanabe K, Iri H
Rinsho Byori. 1989 Jul;37(7):807-12.
We have examined the influence of erythrocytes containing inclusion bodies, nucleated red cells or giant platelets on the measurement of reticulocyte count by automated machine, R-1000. Correlation of the reticulocyte count between automated and conventional method was extremely good in the blood containing red cells with Jolly bodies, Pappenheimer bodies or basophilic stippling . However, correlation was poor when the sample contained the nucleated red cells. Reticulocyte count was decreased in the blood with significant amounts of nucleated red cells. Since nucleated red cells themselves are not counted as reticulocytes in the machine, this was considered to be due to increased young reticulocytes which frequently appeared with nucleated red cells. Both cold agglutinated red cells and giant platelets apparently influenced the reticulocyte count by the R-1000. These results suggest that red cells with Jolly bodies, Pappenheimer bodies or basophilic stippling do not influence the automatic counting of reticulocytes. Although nucleated red cells, cold agglutinated red cells and giant platelets affected the reticulocyte count, the machine shows abnormal flags in most of above cases (except highly agglutinated red cells), so that one can recount reticulocytes by conventional method. We conclude the machine can safely count the reticulocytes even in the blood containing abnormal red cells or platelets.
我们研究了含有包涵体的红细胞、有核红细胞或巨大血小板对自动血细胞分析仪R - 1000检测网织红细胞计数的影响。在含有乔利小体、豪 - 周氏小体或嗜碱性点彩红细胞的血液中，自动检测法与传统方法测得的网织红细胞计数相关性非常好。然而，当样本中含有有核红细胞时，相关性较差。在含有大量有核红细胞的血液中，网织红细胞计数会降低。由于有核红细胞本身在仪器中不被计为网织红细胞，这被认为是由于与有核红细胞同时频繁出现的年轻网织红细胞增多所致。冷凝集红细胞和巨大血小板显然都会影响R - 1000检测网织红细胞计数。这些结果表明，含有乔利小体、豪 - 周氏小体或嗜碱性点彩的红细胞不会影响网织红细胞的自动计数。虽然有核红细胞、冷凝集红细胞和巨大血小板会影响网织红细胞计数，但在大多数上述情况下（高度凝集红细胞除外）仪器会显示异常标记，因此可以用传统方法重新计数网织红细胞。我们得出结论，即使在含有异常红细胞或血小板的血液中，该仪器也能安全地计数网织红细胞。
