Schmitz F J, Werner E
Cytometry. 1986 Sep;7(5):439-44. doi: 10.1002/cyto.990070508.
An automatized technique to count reticulocytes by means of flow cytometry is described. Blood samples were stained by the fluorescent dye acridine orange without the use of fixative. Scatter and red fluorescence of the blood cells were measured in a flow cytometer. A discrimination between reticulocytes and erythrocytes was only achieved by using logarithmic amplification. The discrimination was better in peak mode than in area mode. The optimum dye concentration was 0.5 mg/liter acridine orange. At lower dye concentrations, not all reticulocytes were measured, whereas at higher dye concentrations the degree of discrimination between reticulocytes and erythrocytes decreased. There was a suitable discrimination between reticulocytes and erythrocytes. The reticulocyte numbers were scored by flow cytometry as well as by microscope for blood samples with 0.1-14% reticulocytes. The correlation between both methods was close.
描述了一种通过流式细胞术计数网织红细胞的自动化技术。血液样本用荧光染料吖啶橙染色，无需使用固定剂。在流式细胞仪中测量血细胞的散射光和红色荧光。仅通过对数放大才能实现网织红细胞与红细胞的区分。峰模式下的区分效果优于面积模式。最佳染料浓度为0.5mg/升吖啶橙。染料浓度较低时，并非所有网织红细胞都能被检测到，而染料浓度较高时，网织红细胞与红细胞之间的区分度降低。网织红细胞与红细胞之间存在合适的区分度。对于网织红细胞含量为0.1%-14%的血液样本，通过流式细胞术以及显微镜对网织红细胞数量进行了计数。两种方法之间的相关性密切。
