School of Resource and Environment, Anhui Agricultural University, Hefei, 230036, People's Republic of China.
Environ Sci Pollut Res Int. 2015 Nov;22(21):16535-41. doi: 10.1007/s11356-015-4847-2. Epub 2015 Jun 17.
Effects of various concentrations of Cu(2+) and Zn(2+) (0.0, 0.1, 0.25, 0.5, or 1.0 mg/L) on the growth, malondialdehyde (MDA), the intracellular calcium, and physiological characteristics of green algae, Cladophora, were investigated. Low Zn(2+) concentrations accelerated the growth of Cladophora, whereas Zn(2+) concentration increases to 0.25 mg/L inhibited its growth. Cu(2+) greatly influences Cladophora growth. The photosynthesis of Cladophora decreased under Zn(2+) and Cu(2+) stress. Cu(2+) and Zn(2+) treatment affected the content of total soluble sugar in Cladophora and has small increases in its protein content. Zn(2+) induced the intracellular calcium release, and copper induced the intracellular calcium increases in Cladophora. Exposure to Cu(2+) and Zn(2+) induces MDA in Cladophora. The stress concent of Cu(2+) was strictly correlated with the total soluble sugar content, Chla+Chlb, and MDA in Cladophora, and the stress concent of Zn(2+) was strictly correlated with the relative growth rate (RGR) and MDA of Cladophora.
研究了不同浓度的 Cu(2+) 和 Zn(2+)（0.0、0.1、0.25、0.5 或 1.0mg/L）对绿藻浒苔生长、丙二醛（MDA）、细胞内钙和生理特性的影响。低浓度的 Zn(2+)加速了浒苔的生长，而 Zn(2+)浓度增加到 0.25mg/L 则抑制了其生长。Cu(2+)对浒苔的生长有很大的影响。Zn(2+)和 Cu(2+)胁迫下浒苔的光合作用下降。Cu(2+)和 Zn(2+)处理影响浒苔中总可溶性糖的含量，使其蛋白质含量略有增加。Zn(2+)诱导浒苔细胞内钙释放，铜诱导浒苔细胞内钙增加。Cu(2+)和 Zn(2+)的暴露导致浒苔中 MDA 的产生。Cu(2+)的胁迫浓度与浒苔中的总可溶性糖含量、Chla+Chlb 和 MDA 严格相关，而 Zn(2+)的胁迫浓度与浒苔的相对生长率（RGR）和 MDA 严格相关。
