School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Vegetable Protein Processing Ministry of Education, South China University of Technology, Guangzhou, Guangdong, China.
Department of Civil and Environmental Engineering, University of Maryland, College Park, MD, USA.
Bioengineered. 2022 Mar;13(3):7500-7514. doi: 10.1080/21655979.2022.2047543.
Since antimicrobial resistance, especially β-lactam resistance genes were common in clinical strains, this study had designed and developed multiplex amplification platform for rapid and accurate detection of such resistance genes in 542 clinical isolates. The obtained specimens were subjected to bacteriological examination, antimicrobial susceptibility testing, and detection of β-lactamase genes and plasmid replicons. The major virulence genes were detected by 7 groups of multiplex PCR and eight groups of multiplex PCR were designed to detect 8 different plasmid replicons including , iteron, , and . It was found that most MDR isolates were co-resistant to penicillins (AMP) and fluoroquindones (LVX, CIP) and distribution of LVX and CIP resistance was significantly higher among female than male gender. (AY234375) showed the highest detection rate, followed by the (J01724) and (M26308), indicating the relatively higher carriage rate of corresponding plasmids. acquired the highest carriage rate, followed by group 2 and , indicating their prevalence among clinical . Among the β-lactamase genes, acquired the highest carriage rate, followed by group 2 and , indicating their prevalence among clinical . The (AY234375) showed the highest detection rate, followed by the (J01724) and (M26308), indicating the relatively higher carriage rate of the corresponding plasmids by clinical isolates. It is shown that the developed multiplex amplification methodology is applicable to AMR detection, and such identification of plasmid replicons and β-lactamase genes may aid in the understanding of clinical isolate epidemiology.
由于临床菌株中普遍存在抗菌药物耐药性，尤其是β-内酰胺类耐药基因，本研究设计并开发了一种多重扩增平台，用于快速准确地检测 542 株临床分离株中的这些耐药基因。对获得的标本进行细菌学检查、抗菌药物敏感性试验、β-内酰胺酶基因和质粒复制子检测。采用 7 组多重 PCR 检测主要毒力基因，设计 8 组多重 PCR 检测包括 、iteron 、、在内的 8 种不同质粒复制子。结果发现，大多数 MDR 分离株对青霉素（AMP）和氟喹诺酮类（LVX、CIP）均具有耐药性，且女性对 LVX 和 CIP 的耐药率明显高于男性。（AY234375）检测率最高，其次是 （J01724）和 （M26308），表明相应质粒的携带率相对较高。获得的携带率最高，其次是组 2 和 ，表明其在临床分离株中的流行率较高。在β-内酰胺酶基因中，携带率最高的是组 2 ，其次是 和 ，表明其在临床分离株中较为流行。（AY234375）检测率最高，其次是 （J01724）和 （M26308），表明临床分离株携带相应质粒的比率相对较高。结果表明，所开发的多重扩增方法适用于 AMR 检测，而这种质粒复制子和β-内酰胺酶基因的鉴定可能有助于了解临床分离株的流行病学。
