通过过表达来自短双歧杆菌的α-L-鼠李糖苷酶基因提高人参毛状根中人参皂苷Rg(1)的含量

Enhancement of ginsenoside Rg(1) in Panax ginseng hairy root by overexpressing the α-L-rhamnosidase gene from Bifidobacterium breve.

作者信息

Zhang Ru, Zhang Bian-Ling, Li Gu-Cai, Xie Tao, Hu Teng, Luo Zhi-Yong

机构信息

College of Chemistry and Chemical Engineering, Hunan Institute of Engineering, Xiangtan, 411104, China.

Molecular Biology Research Center, School of Life Sciences, Central South University, Changsha, 410078, China.

出版信息

Biotechnol Lett. 2015 Oct;37(10):2091-6. doi: 10.1007/s10529-015-1889-y. Epub 2015 Jun 19.

DOI:10.1007/s10529-015-1889-y

PMID:26087948

Abstract

OBJECTIVES

To improve the production of ginsenoside Rg1 in Panax ginseng.

RESULTS

The α-L-rhamnosidase gene from Bifidobacterium breve (BbRha) was overexpressed into hairy root culture system using Agrobacterium rhizogenes A4. Ginsenoside Rg1 in hairy roots was obtained following transformation via overexpressed gene representing 2.2-fold higher than those of control lines. Several overexpression transgenic hairy root lines were obtained exhibiting markedly increased levels of the corresponding α-L-rhamnosidase enzymatic activity relative to control. Ginsenoside Rg1 levels in the transgenic lines were higher (2.2-fold) than those of control after following 30 days culturing, while ginsenoside Re contents in tested transgenic lines were found to be lower. The transgenic hairy roots harboring α-L-rhamnosidase gene improved the accumulation of ginsenoside Rg1 up to 3.6 mg g(-1) dry weight.

CONCLUSION

BbRha gene selectively enhances the production of ginsenoside Rg1 in P. ginseng hairy roots.

摘要

目的

提高人参中人参皂苷Rg1的产量。

结果

将来自短双歧杆菌的α-L-鼠李糖苷酶基因（BbRha）通过发根农杆菌A4过表达至毛状根培养系统中。通过过表达基因转化后获得了毛状根中的人参皂苷Rg1，其含量比对照品系高2.2倍。获得了几个过表达转基因毛状根系，相对于对照，其相应的α-L-鼠李糖苷酶活性水平显著提高。培养30天后，转基因品系中的人参皂苷Rg1水平比对照高（2.2倍），而测试的转基因品系中的人参皂苷Re含量较低。携带α-L-鼠李糖苷酶基因的转基因毛状根将人参皂苷Rg1的积累提高至3.6 mg g(-1)干重。