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藏药翼首草正丁醇提取物对Hep3B癌细胞的体内外抗肿瘤作用

In Vitro and In Vivo Antitumor Effects of n-Butanol Extracts of Pterocephalus hookeri on Hep3B Cancer Cell.

作者信息

Guo Chenxu, Wu Yingchun, Zhu Yuanzhang, Wang Yanchun, Tian Lili, Lu Yi, Han Cong, Zhu Guofu

机构信息

School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Department of Traditional Chinese Medicine, Henan Province People's Hospital, Zhengzhou 221000, China.

出版信息

Evid Based Complement Alternat Med. 2015;2015:159132. doi: 10.1155/2015/159132. Epub 2015 May 19.

DOI:10.1155/2015/159132
PMID:26089933
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4452348/
Abstract

Pterocephalus hookeri is a widely applied Tibetan medicinal prescription for treatment of diseases such as flu, rheumatoid arthritis, and enteritis in China. It has been reported that Pterocephalus hookeri has anti-inflammatory and analgesic actions. However, the antitumor activity of Pterocephalus hookeri remains unknown. In the present study, we demonstrate that n-butanol extracts of Pterocephalus hookeri (YSC-ZDC) has a strong antitumor activity against hepatoma carcinoma cell in vitro and in vivo. YSC-ZDC inhibited proliferation of all cancer cell lines and significantly inhibited Hep3B cells proliferation in a dose- and time-dependant manner. Transmission electron microscopy, hoechst 33258 staining, and flow cytometry analysis revealed that YSC-ZDC induced apoptosis in Hep3B cells. YSC-ZDC treatment dramatically inhibited PDK1 and Akt phosphorylation in Hep3B cells. Moreover, YSC-ZDC increased Bax expression and inhibited Bcl-2 expression. In addition, YSC-ZDC inhibited growth hepatoma xenografts in vivo with no effect on body weight and spleen index. Consistent with results in vitro, YSC-ZDC increased Bax expression and inhibited Bcl-2 expression in tumor tissue. Taken together, this study shows YSC-ZDC with an antitumor activity both in vitro and in vivo. Its mechanism underlying is related to blocking of the Akt pathway and regulation of Bcl-2 family proteins expression.

摘要

西藏棱子芹是一种在中国广泛应用于治疗流感、类风湿性关节炎和肠炎等疾病的藏药方剂。据报道，西藏棱子芹具有抗炎和镇痛作用。然而，西藏棱子芹的抗肿瘤活性尚不清楚。在本研究中，我们证明西藏棱子芹正丁醇提取物（YSC-ZDC）在体外和体内对肝癌细胞均具有较强的抗肿瘤活性。YSC-ZDC抑制所有癌细胞系的增殖，并以剂量和时间依赖性方式显著抑制Hep3B细胞的增殖。透射电子显微镜、Hoechst 33258染色和流式细胞术分析表明，YSC-ZDC诱导Hep3B细胞凋亡。YSC-ZDC处理显著抑制Hep3B细胞中PDK1和Akt的磷酸化。此外，YSC-ZDC增加Bax表达并抑制Bcl-2表达。此外，YSC-ZDC在体内抑制肝癌异种移植瘤的生长，对体重和脾脏指数无影响。与体外结果一致，YSC-ZDC增加肿瘤组织中Bax表达并抑制Bcl-2表达。综上所述，本研究表明YSC-ZDC在体外和体内均具有抗肿瘤活性。其潜在机制与阻断Akt通路和调节Bcl-2家族蛋白表达有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d58/4452348/10546ba98730/ECAM2015-159132.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d58/4452348/4689b93e6c0b/ECAM2015-159132.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d58/4452348/f48ad8a6c790/ECAM2015-159132.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d58/4452348/3c2dea84faf3/ECAM2015-159132.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d58/4452348/69cd0edd6fb3/ECAM2015-159132.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d58/4452348/4240aa94368e/ECAM2015-159132.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d58/4452348/828f1c485cf1/ECAM2015-159132.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d58/4452348/10546ba98730/ECAM2015-159132.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d58/4452348/4689b93e6c0b/ECAM2015-159132.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d58/4452348/f48ad8a6c790/ECAM2015-159132.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d58/4452348/3c2dea84faf3/ECAM2015-159132.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d58/4452348/69cd0edd6fb3/ECAM2015-159132.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d58/4452348/4240aa94368e/ECAM2015-159132.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d58/4452348/828f1c485cf1/ECAM2015-159132.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d58/4452348/10546ba98730/ECAM2015-159132.007.jpg

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