Wei Ming-Chi, Yang Yu-Chiao, Hong Show-Jen
Department of Environmental Engineering & Science, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan.
Department and Graduate Institute of Pharmacology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
Evid Based Complement Alternat Med. 2015;2015:450547. doi: 10.1155/2015/450547. Epub 2015 May 18.
Oleanolic acid (OA) and ursolic acid (UA) were extracted from Hedyotis diffusa using a hyphenated procedure of ultrasound-assisted and supercritical carbon dioxide (HSC-CO2) extraction at different temperatures, pressures, cosolvent percentages, and SC-CO2 flow rates. The results indicated that these parameters significantly affected the extraction yield. The maximal yields of OA (0.917 mg/g of dry plant) and UA (3.540 mg/g of dry plant) were obtained at a dynamic extraction time of 110 min, a static extraction time of 15 min, 28.2 MPa, and 56°C with a 12.5% (v/v) cosolvent (ethanol/water = 82/18, v/v) and SC-CO2 flowing at 2.3 mL/min (STP). The extracted yields were then analyzed by high performance liquid chromatography (HPLC) to quantify the OA and UA. The present findings revealed that H. diffusa is a potential source of OA and UA. In addition, using the hyphenated procedure for extraction is a promising and alternative process for recovering OA and UA from H. diffusa at high concentrations.
采用超声辅助与超临界二氧化碳联用（HSC-CO2）萃取法，在不同温度、压力、夹带剂百分比和超临界二氧化碳流速条件下，从白花蛇舌草中提取齐墩果酸（OA）和熊果酸（UA）。结果表明，这些参数对提取率有显著影响。在动态萃取时间110分钟、静态萃取时间15分钟、压力28.2兆帕、温度56℃、夹带剂（乙醇/水=82/18，v/v）含量为12.5%（v/v）以及超临界二氧化碳流速为2.3毫升/分钟（标准温度和压力）的条件下，OA的最大提取率为0.917毫克/克干植物，UA的最大提取率为3.540毫克/克干植物。然后通过高效液相色谱法（HPLC）分析提取率，以定量OA和UA。目前的研究结果表明，白花蛇舌草是OA和UA的潜在来源。此外，采用联用萃取法是从白花蛇舌草中高浓度回收OA和UA的一种有前景的替代方法。
