Department and Graduate Institute of Pharmacology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan; Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan.
Department of Applied Geoinformatics, Chia Nan University of Pharmacy and Science, Tainan 71710, Taiwan.
Food Chem. 2020 Jul 30;319:126582. doi: 10.1016/j.foodchem.2020.126582. Epub 2020 Mar 10.
An ultrasound-assisted supercritical carbon dioxide (USC-CO) procedure was developed for the extraction of ursolic acid, oleanolic acid and oridonin from Rabdosia rubescens, with yields that were 9.84-10.46 and 15.43-21.10% higher than those of the conventional SC-CO and heat-reflux extractions, respectively. USC-CO uses a shorter extraction time (1.83-2.09 times) and less organic solvent (3.39-173.25 times) to operate at a lower extraction temperature (5-16 °C). The dominant component in the extract was oridonin, which may indicate that the kinetic behavior in the extraction system is predominated by that of oridonin. Furthermore, the USC-CO and conventional SC-CO dynamic extraction kinetics of oridonin from R. rubescens were well described by the second-order rate and Fick's second law models. The extraction rate constant, energy of activation for diffusion, Biot number and thermodynamic parameters were deduced from the data obtained. These results provide valuable insights into the USC-CO and conventional SC-CO procedures.
超声辅助超临界二氧化碳（USC-CO）法用于提取冬凌草中的熊果酸、齐墩果酸和冬凌草甲素，与常规的 SC-CO 和热回流提取相比，收率分别提高了 9.84-10.46%和 15.43-21.10%。USC-CO 以较低的提取温度（5-16°C）、更短的提取时间（1.83-2.09 倍）和更少的有机溶剂（3.39-173.25 倍）进行操作。提取物中的主要成分是冬凌草甲素，这可能表明提取体系中的动力学行为主要由冬凌草甲素决定。此外，USC-CO 和常规 SC-CO 从冬凌草中动态提取冬凌草甲素的动力学均能很好地用二级速率和菲克第二定律模型来描述。从获得的数据中推导出了萃取速率常数、扩散活化能、Biot 数和热力学参数。这些结果为 USC-CO 和常规 SC-CO 工艺提供了有价值的见解。
