Xu Chang, Liao Yiyi, Fang Chunyan, Tsunoda Makoto, Zhang Yingxia, Song Yanting, Deng Shiming
Key Laboratory of Tropical Biological Resources of Ministry of Education and Department of Pharmaceutical Sciences, College of Marine Science, Hainan University, Haikou 570228, China.
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
J Anal Methods Chem. 2017;2017:2984562. doi: 10.1155/2017/2984562. Epub 2017 Jul 11.
In this paper, a novel method of QuEChERS-based extraction coupled with high-performance liquid chromatography has been developed for the simultaneous determination of ursolic acid (UA) and oleanolic acid (OA) in guava leaves. The QuEChERS-based extraction parameters, including the amount of added salt, vortex-assisted extraction time, and absorbent amount, and the chromatographic conditions were investigated for the analysis of UA and OA in guava leaves. Under the optimized conditions, the method showed good linearity over a range of 1-320 g mL, with correlation coefficients above 0.999. The limits of detection of UA and OA were 0.18 and 0.36 g mL, respectively. The intraday and interday precision were below 1.95 and 2.55%, respectively. The accuracies of the UA and OA determinations ranged from 97.4 to 111.4%. The contents of UA and OA in the guava leaf samples were 2.50 and 0.73 mg g, respectively. These results demonstrate that the developed method is applicable to the simultaneous determination of UA and OA in guava leaves.
本文建立了一种基于QuEChERS萃取结合高效液相色谱法同时测定番石榴叶中熊果酸（UA）和齐墩果酸（OA）的新方法。研究了基于QuEChERS的萃取参数，包括加盐量、涡旋辅助萃取时间和吸附剂用量，以及用于分析番石榴叶中UA和OA的色谱条件。在优化条件下，该方法在1 - 320μg/mL范围内呈现良好的线性，相关系数高于0.999。UA和OA的检测限分别为0.18和0.36μg/mL。日内和日间精密度分别低于1.95%和2.55%。UA和OA测定的准确度范围为97.4%至111.4%。番石榴叶样品中UA和OA的含量分别为2.50和0.73mg/g。这些结果表明所建立的方法适用于同时测定番石榴叶中的UA和OA。
