Fuentealba Luis C, Rompani Santiago B, Parraguez Jose I, Obernier Kirsten, Romero Ricardo, Cepko Constance L, Alvarez-Buylla Arturo
Department of Neurological Surgery and the Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA 94143, USA.
Departments of Genetics and Ophthalmology and Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA.
Cell. 2015 Jun 18;161(7):1644-55. doi: 10.1016/j.cell.2015.05.041.
Adult neural stem/progenitor (B1) cells within the walls of the lateral ventricles generate different types of neurons for the olfactory bulb (OB). The location of B1 cells determines the types of OB neurons they generate. Here we show that the majority of mouse B1 cell precursors are produced between embryonic days (E) 13.5 and 15.5 and remain largely quiescent until they become reactivated postnatally. Using a retroviral library carrying over 100,000 genetic tags, we found that B1 cells share a common progenitor with embryonic cells of the cortex, striatum, and septum, but this lineage relationship is lost before E15.5. The regional specification of B1 cells is evident as early as E11.5 and is spatially linked to the production of neurons that populate different areas of the forebrain. This study reveals an early embryonic regional specification of postnatal neural stem cells and the lineage relationship between them and embryonic progenitor cells.
侧脑室壁内的成年神经干细胞/祖细胞（B1细胞）为嗅球（OB）生成不同类型的神经元。B1细胞的位置决定了它们所生成的OB神经元的类型。我们在此表明，大多数小鼠B1细胞前体在胚胎期第13.5天至15.5天之间产生，并在很大程度上保持静止，直到出生后被重新激活。通过使用携带超过100,000个基因标签的逆转录病毒文库，我们发现B1细胞与皮质、纹状体和隔膜的胚胎细胞共享一个共同的祖细胞，但这种谱系关系在胚胎期第15.5天之前就已丧失。B1细胞的区域特化早在胚胎期第11.5天就很明显，并且在空间上与填充前脑不同区域的神经元的产生相关联。这项研究揭示了出生后神经干细胞的早期胚胎区域特化以及它们与胚胎祖细胞之间的谱系关系。
