Yilmaz Beyhan, Türkçü Gül, Şengül Engin, Gül Aylin, Özkurt Fazil Emre, Akdağ Mehmet
*Departmant of ENT †Departmant of Pathology, Dicle University Medical School, Diyarbakir, Turkey.
J Craniofac Surg. 2015 Jul;26(5):e422-6. doi: 10.1097/SCS.0000000000001880.
Postoperative nasal mucosa healing is a highly complex and organized process, and the success rates of endoscopic sinus surgery and septoplasty surgeries are closely associated with the postoperative wound healing processes. In this experimental study, the authors' aim was to use histopathologic examination to investigate the effects of N-Acetylcysteine (NAC) on the wound healing of rat nasal mucosa after mechanical trauma. Twenty-one Sprague-Dawley rats were randomly divided into 3 groups: the nontreated group (N = 7), the control saline group (N = 7), and the NAC group (N = 7). No treatment was given to the nontreated group for 15 days. The control saline group received intraperitoneal injection of saline (2.5 mL/kg, intraperitoneal) for 15 days and the NAC group was intraperitoneally injected with NAC at a dose of 300 mg/kg/day for 15 days. At the beginning of the study, unilateral mechanical nasal trauma was induced with an interdental brush inserted through the right nostril in all rats. Samples were stained using hematoxylin and eosin solution, and were examined by a pathologist using a light microscope. The severity of inflammation was milder in the NAC group compared with that in the nontreated and saline groups (P < 0.05). The subepithelial thickness index was lower in the experimental group (P < 0.05). Goblet cell loss was reduced in the experimental group compared with the nontreated and saline groups (P < 0.05). NAC decreases inflammation and goblet cell loss. Therefore, NAC has potential beneficial effects on the wound healing of nasal mucosa in rats.
术后鼻黏膜愈合是一个高度复杂且有组织的过程，鼻内镜鼻窦手术和鼻中隔成形术的成功率与术后伤口愈合过程密切相关。在这项实验研究中，作者的目的是利用组织病理学检查来研究N-乙酰半胱氨酸（NAC）对大鼠鼻黏膜机械创伤后伤口愈合的影响。将21只Sprague-Dawley大鼠随机分为3组：未治疗组（N = 7）、生理盐水对照组（N = 7）和NAC组（N = 7）。未治疗组15天不进行任何治疗。生理盐水对照组腹腔注射生理盐水（2.5 mL/kg，腹腔内注射），持续15天，NAC组腹腔注射剂量为300 mg/kg/天的NAC，持续15天。在研究开始时，所有大鼠均通过将牙间隙刷经右鼻孔插入诱导单侧鼻机械性创伤。样本用苏木精和伊红溶液染色，并由病理学家使用光学显微镜进行检查。与未治疗组和生理盐水组相比，NAC组的炎症严重程度较轻（P < 0.05）。实验组的上皮下厚度指数较低（P < 0.05）。与未治疗组和生理盐水组相比，实验组的杯状细胞损失减少（P < 0.05）。NAC可减轻炎症和杯状细胞损失。因此，NAC对大鼠鼻黏膜伤口愈合具有潜在的有益作用。
