Department of Otolaryngology and Head and Neck Surgery, Ondokuz Mayis University School of Medicine, Tip Fak. KBB Anabilim Dali, Kurupelit, Samsun, 55139, Turkey.
Department of Pharmacology and Toxicology, Ondokuz Mayis University Veterinary Medicine Faculty, Kurupelit, Samsun, 55139, Turkey.
Eur Arch Otorhinolaryngol. 2020 Jun;277(6):1681-1689. doi: 10.1007/s00405-020-05884-1. Epub 2020 Mar 6.
To investigate the effects of submucosal platelet-rich plasma (PRP) injection on nasal mucosal wound healing after endoscopic sinus surgery.
24 New Zealand white rabbits were randomly divided into three groups. To mimic surgery, injury was created using 3-mm punch forceps on ventral turbinate mucosa for all groups. Submucosal PRP was injected to the damaged mucosa in the first group (PRP group, n = 8), where 0.9% saline solution to the second group (saline group, n = 8) and no injection to the third one (control group, n = 8). All the animals were sacrificed 14 days after surgery; histopathological examination and hydroxyproline measurements were performed on the mucosa of all groups.
Neutrophils, goblet cells, and collagen intensity were found significantly lower (p < 0.05) in PRP group than the control group. Also, the number of ciliary and goblet cells, and collagen intensity were found significantly lower (p < 0.05) in PRP group, than the saline group. In PRP group, hydroxyproline levels were found (p < 0.05) significantly lower than the saline and the control group, and no significant difference was found between the saline group and the control group (p > 0.05).
There are many studies in literature showing the positive effects of PRP on wound healing. The results of this study also demonstrated positive effects of PRP on the nasal mucosa. According to these results, PRP injection to the injured nasal mucosa showed anti-inflammatory, mucus-softening, and synechia-reducing effects. Therefore, submucosal PRP injection after endonasal surgeries can be considered an effective application for maintaining nasal physiology.
研究鼻内镜鼻窦手术后黏膜下富血小板血浆（PRP）注射对鼻黏膜创面愈合的影响。
将 24 只新西兰白兔随机分为 3 组。所有组均使用 3-mm 打孔钳对鼻甲腹侧黏膜造成损伤，模拟手术。第一组（PRP 组，n=8）将 PRP 注入受损黏膜下，第二组（盐水组，n=8）注入 0.9%生理盐水，第三组（对照组，n=8）不做任何处理。术后 14 天处死所有动物，对各组黏膜进行组织病理学检查和羟脯氨酸测量。
与对照组相比，PRP 组中性粒细胞、杯状细胞和胶原强度明显降低（p<0.05）。此外，PRP 组的纤毛和杯状细胞数量以及胶原强度明显低于盐水组（p<0.05）。PRP 组羟脯氨酸水平明显低于盐水组和对照组（p<0.05），而盐水组与对照组之间无显著差异（p>0.05）。
文献中有许多研究表明 PRP 对伤口愈合有积极作用。本研究结果也表明 PRP 对鼻黏膜有积极作用。根据这些结果，鼻内镜鼻窦手术后向损伤的鼻黏膜内注射 PRP 可起到抗炎、软化黏液和减少粘连的作用。因此，鼻内镜鼻窦手术后黏膜下 PRP 注射可被视为维持鼻腔生理功能的一种有效应用。
