Kınıs Vefa, Ozbay Musa, Akdag Mehmet, Alabalık Ulas, Gul Aylin, Yılmaz Beyhan, Ozkan Hakan, Topcu Ismail
Department of ENT and Head and Neck Surgery, Dicle University Medical College, Diyarbakir, Turkey.
Department of ENT and Head and Neck Surgery, Dicle University Medical College, Diyarbakir, Turkey.
Am J Otolaryngol. 2014 Jul-Aug;35(4):482-6. doi: 10.1016/j.amjoto.2014.02.008. Epub 2014 Feb 25.
Wound healing of the nasal mucosa is a highly complex process that restores the anatomical and functional integrity of tissue that has been exposed to trauma. In this experimental study, our aim was to use histopathological examination to investigate the effects of caffeic acid phenethyl ester on the wound healing of rat nasal mucosa after mechanical trauma.
The rats were randomly divided into 3 experimental groups: a non-treated group (n=7), a control saline group (n=7) and a caffeic acid phenethyl ester group (n=7). The non-treated group received no treatment for 15 days. The second group was administered saline (2.5 mL/kg, intraperitoneal) once a day for 15 days. The third group received caffeic acid phenethyl ester intraperitoneally at a dose of 10 μmol/kg once a day for 15 days. At the beginning of the study, unilateral mechanical nasal trauma was induced on the right nasal mucosa of all rats in the three groups using a brushing technique. Samples were stained using hematoxylin and eosin solution and were examined by a pathologist using a light microscope.
The severity of inflammation was milder in the caffeic acid phenethyl ester group compared with that in the non-treated and saline groups (P<0.05). The subepithelial thickness index was lower in the experimental group (P<0.05). Goblet cell and ciliated cell loss was substantially reduced in the experimental group compared with the non-treated and saline groups (P<0.05).
Caffeic acid phenethyl ester decreases inflammation and the loss of goblet cells and ciliated cells. Therefore, caffeic acid phenethyl ester has potential beneficial effects on the wound healing of nasal mucosa in the rat.
鼻黏膜伤口愈合是一个高度复杂的过程，可恢复遭受创伤组织的解剖和功能完整性。在本实验研究中，我们的目的是利用组织病理学检查来研究咖啡酸苯乙酯对大鼠鼻黏膜机械创伤后伤口愈合的影响。
将大鼠随机分为3个实验组：未处理组（n = 7）、生理盐水对照组（n = 7）和咖啡酸苯乙酯组（n = 7）。未处理组15天不进行处理。第二组每天腹腔注射生理盐水（2.5 mL/kg），共15天。第三组每天腹腔注射剂量为10 μmol/kg的咖啡酸苯乙酯，共15天。在研究开始时，使用刷涂技术对三组所有大鼠的右侧鼻黏膜造成单侧机械性鼻创伤。样本用苏木精和伊红溶液染色，并由病理学家使用光学显微镜进行检查。
与未处理组和生理盐水组相比，咖啡酸苯乙酯组的炎症严重程度较轻（P<0.05）。实验组的上皮下厚度指数较低（P<0.05）。与未处理组和生理盐水组相比，实验组杯状细胞和纤毛细胞的损失明显减少（P<0.05）。
咖啡酸苯乙酯可减轻炎症以及杯状细胞和纤毛细胞的损失。因此，咖啡酸苯乙酯对大鼠鼻黏膜伤口愈合具有潜在的有益作用。
