Nanomedicine Research Laboratory, Medical Intensive Care Clinic, University Hospital Basel, Basel, Switzerland.
Nanomedicine Research Laboratory, Medical Intensive Care Clinic, University Hospital Basel, Basel, Switzerland; CLINAM-European Foundation for Clinical Nanomedicine, Basel, Switzerland.
Nanomedicine. 2015 Oct;11(7):1745-61. doi: 10.1016/j.nano.2015.05.009. Epub 2015 Jun 17.
Due to the progressive spread of the dengue virus and a rising incidence of dengue disease, its rapid diagnosis is important for developing countries and of increasing relevance for countries in temperate climates. Recent advances in bioelectronics, micro- and nanofabrication technologies have led to new miniaturized point-of-care devices and analytical platforms suited for rapid detection of infections. Starting from the available tests for dengue diagnosis, this review examines emerging rapid, micro/nanotechnologies-based tools, including label-free biosensor methods, microarray and microfluidic platforms, which hold significant potential, but still need further development and evaluation. The epidemiological and clinical setting as key determinants for selecting the best analytical strategy in patients presenting with fever is then discussed. This review is aimed at the clinicians and microbiologists to deepen understanding and enhance application of dengue diagnostics, and also serves as knowledge base for researchers and test developers to overcome the challenges posed by this disease.
Dengue disease remains a significant problem in many developing countries. Unfortunately rapid diagnosis with easy and low cost tests for this disease is currently still not realized. In this comprehensive review, the authors highlighted recent advances in nanotechnology which would enable development in this field, which would result in beneficial outcomes to the population.
由于登革热病毒的不断传播和登革热发病率的上升，快速诊断对发展中国家很重要，对温带气候国家的相关性也越来越大。生物电子学、微纳制造技术的最新进展导致了适合快速检测感染的新型微型即时检测设备和分析平台。本综述从现有的登革热诊断测试开始，检查了新兴的快速、基于微/纳米技术的工具，包括无标记生物传感器方法、微阵列和微流控平台，这些工具具有很大的潜力，但仍需要进一步的开发和评估。然后讨论了在出现发热的患者中选择最佳分析策略的关键决定因素，包括流行病学和临床环境。本综述的目的是为临床医生和微生物学家提供更深入的了解，并增强对登革热诊断的应用，也为研究人员和测试开发者提供了一个知识库，以克服该疾病带来的挑战。
登革热疾病仍然是许多发展中国家的一个重大问题。不幸的是，目前仍未实现用简便、低成本的检测方法快速诊断这种疾病。在这篇全面的综述中，作者强调了纳米技术的最新进展，这将促进该领域的发展，从而使人们受益。
