Lee Jong Ho, Shin Yong Cheol, Jin Oh Seong, Kang Seok Hee, Hwang Yu-Shik, Park Jong-Chul, Hong Suck Won, Han Dong-Wook
Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, Korea.
Nanoscale. 2015 Jul 21;7(27):11642-51. doi: 10.1039/c5nr01580d. Epub 2015 Jun 22.
Human mesenchymal stem cells (hMSCs) have great potential as cell sources for bone tissue engineering and regeneration, but the control and induction of their specific differentiation into bone cells remain challenging. Graphene-based nanomaterials are considered attractive candidates for biomedical applications such as scaffolds in tissue engineering, substrates for SC differentiation and components of implantable devices, due to their biocompatible and bioactive properties. Despite the potential biomedical applications of graphene and its derivatives, only limited information is available regarding their osteogenic activity. This study concentrates upon the effects of reduced graphene oxide (rGO)-coated hydroxyapatite (HAp) composites on osteogenic differentiation of hMSCs. The average particle sizes of HAp and rGO were 1270 ± 476 nm and 438 ± 180 nm, respectively. When coated on HAp particulates, rGO synergistically enhanced spontaneous osteogenic differentiation of hMSCs, without hampering their proliferation. This result was confirmed by determining alkaline phosphatase activity and mineralization of calcium and phosphate as early and late stage markers of osteogenic differentiation. It is suggested that rGO-coated HAp composites can be effectively utilized as dental and orthopedic bone fillers since these graphene-based particulate materials have potent effects on stimulating the spontaneous differentiation of MSCs and show superior bioactivity and osteoinductive potential.
人骨髓间充质干细胞（hMSCs）作为骨组织工程和再生的细胞来源具有巨大潜力，但对其向骨细胞的特异性分化进行控制和诱导仍然具有挑战性。基于石墨烯的纳米材料因其生物相容性和生物活性特性，被认为是生物医学应用的有吸引力的候选材料，如组织工程中的支架、干细胞分化的基质和可植入设备的组件。尽管石墨烯及其衍生物具有潜在的生物医学应用，但关于它们的成骨活性的信息有限。本研究集中于还原氧化石墨烯（rGO）包覆的羟基磷灰石（HAp）复合材料对hMSCs成骨分化的影响。HAp和rGO的平均粒径分别为1270±476nm和438±180nm。当rGO包覆在HAp颗粒上时，可协同增强hMSCs的自发成骨分化，而不影响其增殖。通过测定碱性磷酸酶活性以及钙和磷的矿化情况（作为成骨分化的早期和晚期标志物）证实了这一结果。提示rGO包覆的HAp复合材料可有效用作牙科和矫形骨填充材料，因为这些基于石墨烯的颗粒材料对刺激间充质干细胞的自发分化具有显著作用，并显示出优异的生物活性和骨诱导潜力。
