Wang Junjian, Chen Peng, Xu Jianzhen, Zou June X, Wang Haibin, Chen Hong-Wu
Comprehensive Cancer Center, University of California Davis, Sacramento, California, 95817, United States of America; Department of Biochemistry and Molecular Medicine, School of Medicine, University of California Davis, Sacramento, California, 95817, United States of America.
Comprehensive Cancer Center, University of California Davis, Sacramento, California, 95817, United States of America; First Clinical Medicine School of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
PLoS One. 2015 Jun 22;10(6):e0128229. doi: 10.1371/journal.pone.0128229. eCollection 2015.
Mammalian cells including human cancer cells are usually transported in cryovials on dry ice or in a liquid nitrogen vapor shipping vessel between different places at long distance. The hazardous nature of dry ice and liquid nitrogen, and the associated high shipping cost strongly limit their routine use. In this study, we tested the viability and properties of cells after being preserved or shipped over long distance in Matrigel mixture for different days. Our results showed that cells mixed with Matrigel at suitable ratios maintained excellent viability (>90%) for one week at room temperature and preserved the properties such as morphology, drug sensitivity and metabolism well, which was comparable to cells cryopreserved in liquid nitrogen. We also sent cells in the Matrigel mixture via FedEx service to different places at ambient temperature. Upon arrival, it was found that over 90% of the cells were viable and grew well after replating. These data collectively suggested that our Matrigel-based method was highly convenient for shipping live cells for long distances in semi-solid gel condition and at ambient temperature.
包括人类癌细胞在内的哺乳动物细胞通常在干冰上的冻存管中或在液氮蒸汽运输容器中长距离运输到不同地点。干冰和液氮的危险性以及相关的高昂运输成本严重限制了它们的常规使用。在本研究中，我们测试了细胞在不同天数于基质胶混合物中保存或长距离运输后的活力和特性。我们的结果表明，以合适比例与基质胶混合的细胞在室温下一周内保持了优异的活力（>90%），并且很好地保留了形态、药物敏感性和代谢等特性，这与在液氮中冷冻保存的细胞相当。我们还通过联邦快递服务在环境温度下将基质胶混合物中的细胞送到不同地点。到达后发现，超过90%的细胞活力良好，重新接种后生长良好。这些数据共同表明，我们基于基质胶的方法在半固体凝胶状态下和环境温度下长距离运输活细胞非常方便。
