Yang Lingzhi, Li Chufang, Chen Ling, Li Zhiyuan
State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences (GIBH), Guangzhou 510663, China.
Curr Chem Genomics. 2009 Dec 16;3:50-3. doi: 10.2174/1875397300903010050.
Cryopreserved cells stored in dry ice or liquid nitrogen is the classical method for transporting cells between research laboratories in different cities around the world in order to maintain cell viability. An alternative method is to ship the live cells in flasks filled with cell culture medium. Both methods have limitations of either a requirement on special shipping container or short times for the cells to survive on the shipping process. We have recently developed an agarose gel based method for directly transporting the live adherent cells in cell culture plates or dishes in ambient temperature. This convenient method simplifies the transportation of live cells in long distance that can maintain cells in good viability for several days.
保存在干冰或液氮中的冷冻保存细胞是在世界各地不同城市的研究实验室之间运输细胞以维持细胞活力的经典方法。另一种方法是在装满细胞培养基的培养瓶中运输活细胞。这两种方法都有局限性，要么需要特殊的运输容器，要么细胞在运输过程中的存活时间较短。我们最近开发了一种基于琼脂糖凝胶的方法，用于在室温下直接在细胞培养板或培养皿中运输贴壁活细胞。这种便捷的方法简化了活细胞的长途运输，可使细胞在数天内保持良好的活力。
