Meemon Krai, Sobhon Prasert
Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Rd., Bangkok, 10400, Thailand,
Parasitol Res. 2015 Aug;114(8):2807-13. doi: 10.1007/s00436-015-4589-6. Epub 2015 Jun 24.
Fasciolosis, caused by Fasciola hepatica and Fasciola gigantica, is one of the most neglected tropical zoonotic diseases. One sustainable control strategy against these infections is the employment of vaccines that target proteins essential for parasites' invasion and nutrition acquiring processes. Cathepsin proteases are the most abundantly expressed proteins in Fasciola spp. that have been tested successfully as vaccines against fasciolosis in experimental as well as large animals because of their important roles in digestion of nutrients, invasion, and migration. Specifically, juvenile-specific cathepsin proteases are the more effective vaccines because they could block the invasion and migration of juvenile parasites whose immune evasion mechanism has not yet been fully developed. Moreover, because of high sequence similarity and identity of cathepsins from juveniles with those of adults, the vaccines can attack both the juvenile and adult stages. In this article, the characteristics and vaccine potentials of juvenile-specific cathepsins, i.e., cathepsins L and B, of Fasciola spp. were reviewed.
由肝片吸虫和巨片吸虫引起的片形吸虫病是最被忽视的热带人畜共患病之一。针对这些感染的一种可持续控制策略是使用针对寄生虫入侵和营养获取过程所必需蛋白质的疫苗。组织蛋白酶是片形吸虫属中表达最丰富的蛋白质，由于它们在营养消化、入侵和迁移中发挥重要作用，已在实验动物和大型动物中作为抗片形吸虫病疫苗进行了成功测试。具体而言，幼虫特异性组织蛋白酶是更有效的疫苗，因为它们可以阻断幼虫寄生虫的入侵和迁移，而幼虫寄生虫的免疫逃避机制尚未完全发育。此外，由于幼虫组织蛋白酶与成虫组织蛋白酶具有高度的序列相似性和同一性，这些疫苗可以同时攻击幼虫和成虫阶段。本文综述了片形吸虫属幼虫特异性组织蛋白酶，即组织蛋白酶L和B的特性及疫苗潜力。
