将尼尔森-索莫吉还原糖测定法改编为使用微量滴定板的微量测定法。
Adaptation of the Nelson-Somogyi reducing-sugar assay to a microassay using microtiter plates.
作者信息
Green F, Clausen C A, Highley T L
机构信息
USDA Forest Service, Forest Products Laboratory, Madison, Wisconsin 53705-2398.
出版信息
Anal Biochem. 1989 Nov 1;182(2):197-9. doi: 10.1016/0003-2697(89)90578-2.
PMID:2610335
Abstract
The Nelson-Somogyi assay for reducing sugars was adapted to microtiter plates. The primary advantages of this modified assay are (i) smaller sample and reagent volumes, (ii) elimination of boiling and filtration steps, (iii) automated measurement with a dual-wavelength scanning TLC densitometer, (iv) increased range and reproducibility, and (v) automated colorimetric readings by reflectance rather than absorbance.
摘要
用于还原糖检测的尼尔森 - 索莫吉法被应用于微量滴定板。这种改良检测方法的主要优点包括：（i）样品和试剂体积更小；（ii）无需煮沸和过滤步骤；（iii）使用双波长扫描薄层色谱密度计进行自动测量；（iv）检测范围扩大且重现性提高；（v）通过反射率而非吸光度进行比色法自动读数。
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