通过使用微量滴定板使索莫吉-尼尔森测定法小型化来改进还原糖的定量分析。
Improvement in the quantification of reducing sugars by miniaturizing the Somogyi-Nelson assay using a microtiter plate.
作者信息
Shao Yijing, Lin Amy Hui-Mei
机构信息
Bi-State School of Food Science, University of Idaho and Washington State University, 875 Perimeter Dr. MS 2312, Moscow, ID 83843-2312, USA.
出版信息
Food Chem. 2018 Feb 1;240:898-903. doi: 10.1016/j.foodchem.2017.07.083. Epub 2017 Jul 21.
Measuring reducing sugar is a common practice in carbohydrate research, and the colorimetric assay developed by Somogyi and Nelson has a high sensitivity in a broad concentration range. However, the method is time-consuming when analyzing a large number of samples. In this study, a modified Somogyi-Nelson assay with excellent accuracy and sensitivity was developed using a 96-well microplate. This microassay greatly improves the analytic capacity and efficacy of the method.
测定还原糖是碳水化合物研究中的常见操作，由索莫吉和纳尔逊开发的比色测定法在很宽的浓度范围内具有高灵敏度。然而，该方法在分析大量样品时很耗时。在本研究中，使用96孔微孔板开发了一种具有优异准确性和灵敏度的改良索莫吉-纳尔逊测定法。这种微量测定法极大地提高了该方法的分析能力和效率。
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