Hammond A C, Allison M J, Williams M J, Prine G M, Bates D B
Agricultural Research Service, Subtropical Agricultural Research Station, Brooksville, FL 34605.
Am J Vet Res. 1989 Dec;50(12):2176-80.
Ruminal microorganisms in cattle at a Florida agriculture research station did not have the ability to detoxify leucaena by degradation of 3-hydroxy-4(1H)-pyridone (3,4,-DHP), but a DHP isomer (2,3-DHP) was degraded in some cattle. Cattle with microorganisms that degraded 2,3-DHP were mostly Senepol cattle imported from St. Croix, US Virgin Islands, where leucaena is an indigenous species. Hereford cattle at the research station in Florida generally did not degrade 3,4-DHP or 2,3-DHP. An experiment was conducted in which a pure culture of 3,4-DHP-degrading bacteria was inoculated into Hereford cattle (with ruminal fistula) grazing leucaena. The bacteria successfully colonized the rumen of recipient cattle and persisted through the following winter when there was no leucaena in the diet.
佛罗里达农业研究站的牛瘤胃微生物没有通过降解3-羟基-4(1H)-吡啶酮（3,4-DHP）来解毒银合欢的能力，但在一些牛中，一种DHP异构体（2,3-DHP）被降解了。具有降解2,3-DHP微生物的牛大多是从美属维尔京群岛圣克罗伊岛进口的塞内波尔牛，在那里银合欢是本土物种。佛罗里达研究站的赫里福德牛通常不会降解3,4-DHP或2,3-DHP。进行了一项实验，将一种降解3,4-DHP的纯培养细菌接种到放牧银合欢的有瘤胃瘘管的赫里福德牛体内。这些细菌成功地在受体牛的瘤胃中定殖，并在接下来的冬季饲料中没有银合欢时持续存在。
