McSweeny C S, Allison M J, Mackie R I
CSIRO Division of Tropical Animal Production, Long Pocket Laboratories, Indooroopilly, Qld., Australia.
Arch Microbiol. 1993;159(2):131-5. doi: 10.1007/BF00250272.
The ruminal bacterium Synergistes jonesii strain 78-1, which is able to degrade the pyridinediol toxin in the plant Leucaena leucocephala, was studied for its ability to utilise amino acids. The organism used arginine, histidine and glycine from a complex mixture of amino acids, and both arginine and histidine supported growth in a semi-defined medium. The products of (U-14C)-arginine metabolism were CO2 acetate, butyrate, citrulline and ornithine. The labelling pattern of end products from (U-14C)-histidine metabolism differed in that carbon also flowed into formate and propionate. Arginine was catabolised by the arginine deiminase pathway which was characterised by the presence of arginine deiminase, ornithine transcarbamylase and carbamate kinase. This is the first report of a rumen bacterium that uses arginine and histidine as major energy yielding substrates.
瘤胃细菌琼斯氏协同杆菌78-1菌株能够降解银合欢属植物中的吡啶二醇毒素，对其利用氨基酸的能力进行了研究。该微生物能从复杂的氨基酸混合物中利用精氨酸、组氨酸和甘氨酸，并且精氨酸和组氨酸都能在半限定培养基中支持其生长。（U-14C）-精氨酸代谢的产物是二氧化碳、乙酸、丁酸、瓜氨酸和鸟氨酸。（U-14C）-组氨酸代谢终产物的标记模式有所不同，因为碳还流入甲酸和丙酸。精氨酸通过精氨酸脱亚氨酶途径进行分解代谢，该途径的特征是存在精氨酸脱亚氨酶、鸟氨酸转氨甲酰酶和氨基甲酸激酶。这是关于瘤胃细菌将精氨酸和组氨酸用作主要产能量底物的首次报道。
