Chowdhury Mohammed Riaz Hasan, Sagor Md Abu Taher, Tabassum Nabila, Potol Md Abdullah, Hossain Hemayet, Alam Md Ashraful
Department of Pharmaceutical Sciences, North South University, Bashundhara, Dhaka 1229, Bangladesh.
BCSIR Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka 1205, Bangladesh.
Evid Based Complement Alternat Med. 2015;2015:598179. doi: 10.1155/2015/598179. Epub 2015 May 28.
Citrus maxima peel is rich in natural phenolic compounds and has a long use in the traditional medicine. HPLC-DAD analysis on Citrus maxima peel powder exhibited the presence of various phenolic compounds such as caffeic acid and (-)-epicatechin. To determine the plausible hepatoprotective activity of Citrus maxima peel powder, we used carbon tetrachloride (CCl4) treated rat model. Liver damage in rats was confirmed by measuring the AST, ALT, and ALP enzyme activities. In addition, lipid peroxidation products (MDA), nitric oxide, advanced protein oxidation products level (APOP), and catalase activities were also analyzed along with the histological profiling for the inflammatory cell infiltration, collagen, and iron deposition in liver. Dietary supplementation of Citrus maxima peel powder exhibited significant reduction of serum AST, ALT, and ALP activities in carbon tetrachloride treated rats. Moreover, Citrus maxima peel powder also showed a significant reduction of the oxidative stress markers (MDA, NO, and APOP level) and restored the catalase activity in CCl4 treated rats. Histological examination of the liver section revealed reduced inflammatory cells infiltration, collagen, and iron deposition in CCl4 treated rats. The results from this study demonstrated that Citrus maxima peel powder produced significant hepatoprotective action in CCl4 administered rats.
柚皮富含天然酚类化合物，在传统医学中有着悠久的应用历史。对柚皮粉末进行的高效液相色谱-二极管阵列检测（HPLC-DAD）分析显示，其中存在多种酚类化合物，如咖啡酸和（-）-表儿茶素。为了确定柚皮粉末可能具有的肝保护活性，我们使用了四氯化碳（CCl4）处理的大鼠模型。通过测量天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和碱性磷酸酶（ALP）的酶活性来确认大鼠的肝损伤。此外，还分析了脂质过氧化产物（MDA）、一氧化氮、晚期蛋白质氧化产物水平（APOP）和过氧化氢酶活性，并对肝脏中的炎性细胞浸润、胶原蛋白和铁沉积进行了组织学分析。在四氯化碳处理的大鼠中，饮食补充柚皮粉末可显著降低血清AST、ALT和ALP的活性。此外，柚皮粉末还显著降低了氧化应激标志物（MDA、NO和APOP水平），并恢复了四氯化碳处理大鼠的过氧化氢酶活性。肝脏切片的组织学检查显示，四氯化碳处理大鼠的炎性细胞浸润、胶原蛋白和铁沉积减少。本研究结果表明，柚皮粉末对四氯化碳给药的大鼠具有显著的肝保护作用。
