Department of Pharmacology, Lovely Professional University, Jalandhar, Punjab 144411, India.
Department of Pharmacology, Swift School of Pharmacy, Rajpura, Punjab 140401, India.
Biomed Res Int. 2022 Jun 16;2022:2807337. doi: 10.1155/2022/2807337. eCollection 2022.
Diabetic cardiomyopathy (DCM) pathogenesis is multifarious, and there are insufficient therapeutic options to treat DCM. The present research explored the effects of Citrus grandis peel ethanolic extract (CGPE) in alloxan-induced DCM in rats. Diabetes was triggered by intraperitoneal (i.p.) injection of alloxan (150 mg/kg) in Wistar rats (200-250 g). CGPE (100, 200, and 400 mg/kg) or glibenclamide (Glib, 10 mg/kg) were administered orally for 2 weeks. After the treatment schedule, prooxidants (thiobarbituric acid reactive substances), antioxidants (glutathione, catalase, and superoxide dismutase), and inflammatory markers (tumor necrosis factor-) were determined in cardiac tissues. Biomarkers of cell death, viz., lactate dehydrogenase (LDH), creatine kinase MB (CK-MB) activity, glucose levels, total cholesterol (TC), and high-density lipoproteins (HDL), were assessed in the blood. Rats administered with alloxan showed a consistent increase in blood glucose level (days 7 and 14) that was lowered considerably ( < 0.001) by CGPE or Glib. Alloxan-induced increase in LDH, CK-MB, TC, and decline in HDL was attenuated ( < 0.001) in rats that were treated with CGPE or Glib. Alloxan significantly ( < 0.001) elevated oxidative stress, inflammation, and reduced antioxidants in the cardiac tissue of rats, and these pathogenic abnormalities were ameliorated ( < 0.001) by CGPE. Histopathological studies showed a decrease in morphological disruptions by alloxan in CGPE-treated rats. CGPE (400 mg/kg) significantly ameliorated biochemical parameters in comparison to the lower doses against alloxan cardiotoxicity. Citrus grandis peel extract can be an alternative in the management of DCM.
糖尿病心肌病（DCM）的发病机制多种多样，目前治疗 DCM 的方法选择有限。本研究探讨了柚皮乙醇提取物（CGPE）在链脲佐菌素诱导的大鼠 DCM 中的作用。通过腹腔内（i.p.）注射链脲佐菌素（150mg/kg）诱导 Wistar 大鼠（200-250g）糖尿病。CGPE（100、200 和 400mg/kg）或格列本脲（Glib，10mg/kg）经口给药 2 周。治疗方案结束后，测定心脏组织中的促氧化剂（硫代巴比妥酸反应物质）、抗氧化剂（谷胱甘肽、过氧化氢酶和超氧化物歧化酶）和炎症标志物（肿瘤坏死因子-α）。测定血液中的细胞死亡生物标志物，即乳酸脱氢酶（LDH）、肌酸激酶 MB（CK-MB）活性、血糖水平、总胆固醇（TC）和高密度脂蛋白（HDL）。用链脲佐菌素处理的大鼠血糖水平持续升高（第 7 天和第 14 天），CGPE 或格列本脲可显著降低血糖水平（<0.001）。CGPE 或格列本脲可减轻链脲佐菌素诱导的 LDH、CK-MB、TC 升高和 HDL 降低（<0.001）。链脲佐菌素显著（<0.001）增加了大鼠心脏组织的氧化应激、炎症和抗氧化剂减少，CGPE 可改善这些病理异常（<0.001）。组织病理学研究显示，CGPE 处理的大鼠心脏组织形态破坏减少。与较低剂量相比，CGPE（400mg/kg）可显著改善对链脲佐菌素心脏毒性的生化参数。柚皮提取物可作为 DCM 治疗的一种替代方法。
