一种海洋藻类提取物的抗真菌活性筛选

Screening of a Marine Algal Extract for Antifungal Activities.

作者信息

Lopes Graciliana, Andrade Paula B, Valentão Patrícia

机构信息

REQUIMTE/Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge de Viterbo Ferreira 228, 4050-313, Porto, Portugal.

出版信息

Methods Mol Biol. 2015;1308:411-20. doi: 10.1007/978-1-4939-2684-8_26.

DOI:10.1007/978-1-4939-2684-8_26

PMID:26108521

Abstract

Over the past few years algal extracts have become increasingly interesting to the scientific community due to their promising biological properties. Phlorotannin extracts are particularly attractive partly due to their reported antifungal activity against several yeast and dermatophyte strains.The micromethod used for the evaluation of the minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) represents an effective and solvent-saving procedure to evaluate the antifungal activity of algae extracts. Here we describe the micromethod for determining the MIC and the MLC of algal extracts by using the example of a purified phlorotannin extract of brown algae.

摘要

在过去几年中，藻类提取物因其具有前景的生物学特性而越来越受到科学界的关注。褐藻多酚提取物尤其具有吸引力，部分原因是据报道它们对多种酵母和皮肤癣菌菌株具有抗真菌活性。用于评估最低抑菌浓度（MIC）和最低致死浓度（MLC）的微量法是一种评估藻类提取物抗真菌活性的有效且节省溶剂的方法。在此，我们以一种纯化的褐藻褐藻多酚提取物为例，描述测定藻类提取物MIC和MLC的微量法。