一种用于大规模蛋白质组学中高效电子转移解离（ETD）的校准程序。
A calibration routine for efficient ETD in large-scale proteomics.
作者信息
Rose Christopher M, Rush Matthew J P, Riley Nicholas M, Merrill Anna E, Kwiecien Nicholas W, Holden Dustin D, Mullen Christopher, Westphall Michael S, Coon Joshua J
机构信息
Department of Chemistry, University of Wisconsin, Madison, WI, 53706, USA.
Genome Center of Wisconsin, University of Wisconsin, Madison, WI, 53706, USA.
出版信息
J Am Soc Mass Spectrom. 2015 Nov;26(11):1848-57. doi: 10.1007/s13361-015-1183-1. Epub 2015 Jun 26.
Electron transfer dissociation (ETD) has been broadly adopted and is now available on a variety of commercial mass spectrometers. Unlike collisional activation techniques, optimal performance of ETD requires considerable user knowledge and input. ETD reaction duration is one key parameter that can greatly influence spectral quality and overall experiment outcome. We describe a calibration routine that determines the correct number of reagent anions necessary to reach a defined ETD reaction rate. Implementation of this automated calibration routine on two hybrid Orbitrap platforms illustrate considerable advantages, namely, increased product ion yield with concomitant reduction in scan rates netting up to 75% more unique peptide identifications in a shotgun experiment. Graphical Abstract ᅟ.
电子转移解离（ETD）已被广泛采用，现在各种商用质谱仪上均可使用。与碰撞激活技术不同，ETD的最佳性能需要用户具备相当多的知识并进行大量投入。ETD反应持续时间是一个关键参数，它会极大地影响光谱质量和整体实验结果。我们描述了一种校准程序，该程序可确定达到定义的ETD反应速率所需的正确试剂阴离子数量。在两个混合轨道阱平台上实施这种自动校准程序显示出相当大的优势，即提高了产物离子产率，同时降低了扫描速率，在鸟枪法实验中净增加了多达75%的独特肽段鉴定。图形摘要ᅟ 。
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