内镜手术下真菌团块培养分析真菌球性鼻窦炎
Analysis of fungal ball rhinosinusitis by culturing fungal clumps under endoscopic surgery.
作者信息
Zhang Junyi, Li Yunchuan, Lu Xinxin, Wang Xiangdong, Zang Hongrui, Wang Tong, Zhou Bing, Zhang Luo
机构信息
Department of Otolaryngology Head and Neck Surgery, State Key Laboratory Otolaryngology Head and Neck Surgery of Ministry of Education, Beijing Tongren Hospital, Capital Medical University Beijing 100730, China ; Department of Otolaryngology, Daqing Oilfield General Hospital Daqing 163001, Heilongjiang Province, China.
Department of Otolaryngology Head and Neck Surgery, State Key Laboratory Otolaryngology Head and Neck Surgery of Ministry of Education, Beijing Tongren Hospital, Capital Medical University Beijing 100730, China.
出版信息
Int J Clin Exp Med. 2015 Apr 15;8(4):5925-30. eCollection 2015.
OBJECTIVE
This study was designed to investigate the clinical microbiology of fungal ball (FB) rhinosinusitis by culturing fungal clumps collected under endoscopic surgery.
METHODS
From April to November of 2012, fungal clumps were sampled by endoscopic surgery from patients diagnosed with FB using clinical and histopathological methods. The specimens were subjected to smear microscopy, and cultured for bacteria and fungi analysis.
RESULTS
Out of the 81 specimens from 80 patients, 69 (69/81, 85.19%) specimens were detected a mixed infection of bacteria and fungi. However, only 25 (25/81, 30.86%) specimens resulted in fungal growth. There were 12 (12/81, 14.81%) specimens with fungal infections alone. The cultured fungi included 36 strains belonging to five genera, and most of them were Aspergillus spp. (30/36, 83.3%). The cultured bacteria included 94 strains belonging to 16 genera, and the most frequently seen was Staphylococcus spp. (23/94, 35.94%). When it was fungal and Pseudomonas aeruginosa mixed infection, the fungal growth was inhibited (P = 0.002).
CONCLUSION
Patients with fungal ball usually have mixed fungal and bacterial infections. The fungi from these samples are sometimes difficult to culture, which may be the result of the inhibition by bacteria in vitro and in vivo.
目的
本研究旨在通过培养在内镜手术中采集的真菌团块，调查真菌球型鼻窦炎的临床微生物学情况。
方法
2012年4月至11月，采用临床和组织病理学方法，对诊断为真菌球型鼻窦炎的患者进行内镜手术采集真菌团块。对标本进行涂片显微镜检查，并进行细菌和真菌培养分析。
结果
在80例患者的81份标本中，69份（69/81，85.19%）标本检测到细菌和真菌混合感染。然而，仅25份（25/81，30.86%）标本培养出真菌生长。有12份（12/81，14.81%）标本为单纯真菌感染。培养出的真菌包括属于5个属的36株，其中大部分为曲霉菌属（30/36，83.3%）。培养出的细菌包括属于16个属的94株，最常见的是葡萄球菌属（23/9
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