National Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.
College of Agronomy, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi, Xinjiang, 830052, China.
Theor Appl Genet. 2015 Oct;128(10):2067-76. doi: 10.1007/s00122-015-2567-0. Epub 2015 Jul 2.
Three novel QTL for peroxidase activity were mapped, and gene-specific markers for TaPod-A1 were developed and validated using RILs derived from the Doumai/Shi 4185 cross and 281 wheat cultivars. TaPod-A1 is within one of the three QTL. Peroxidase (POD) activity in grain is an important factor determining the color of flour and end-use products of wheat, such as noodles and steamed bread. Mapping QTL for POD activity, characterization of POD genes and development of gene-specific markers are important for molecular marker-assisted selection in wheat breeding. Quantitative trait loci (QTL) for POD activity in common wheat were mapped using a recombinant inbred line (RIL) population derived from a Doumai/Shi 4185 cross grown in four environments and genotyped using the wheat 90 K iSelect assay. Three novel QTL for POD activity, QPod.caas-3AL, QPod.caas-4BS and QPod.caas-5AS, were identified on chromosomes 3AL, 4BS and 5AS, explaining 5.3-21.2% of phenotypic variance across environments. The full-length genomic DNA (gDNA) sequence of a POD gene, designated TaPod-A1, on chromosome 3A was characterized by homolog cloning and PCR verification. Two complementary dominant sequence-tagged site (STS) markers, POD-3A1 and POD-3A2, were developed based on single nucleotide polymorphisms (SNPs) between two alleles at the TaPod-A1 locus, amplifying 291- and 766-bp fragments in cultivars with lower and higher POD activities, respectively. The two gene-specific markers were mapped on chromosome 3AL using a set of Chinese Spring (CS) nulli-tetrasomic lines, and ditelosomic lines 3AL and 3AS. QTL analysis indicated that QPod.caas-3AL co-segregated with the gene-specific markers POD-3A1 and POD-3A2. POD-3A1 and POD-3A2 were verified on 281 wheat cultivars and advanced lines, and showed significant (P < 0.05) associations with POD activities. POD-3A1 and POD-3A2 may be useful as markers for improving color attributes in wheat breeding programs.
三个新的过氧化物酶活性 QTL 被定位，并且使用来自 Doumai/Shi 4185 杂交和 281 个小麦品种的 RIL 开发和验证了 TaPod-A1 的基因特异性标记。TaPod-A1 位于三个 QTL 之一。籽粒中的过氧化物酶（POD）活性是决定面粉颜色和小麦最终用途产品（如面条和馒头）的重要因素。定位 POD 活性的 QTL、鉴定 POD 基因和开发基因特异性标记对于小麦育种中的分子标记辅助选择非常重要。使用来自 Doumai/Shi 4185 杂交的重组自交系（RIL）群体在四个环境中生长，并使用小麦 90 K iSelect 测定进行基因型分析，定位了普通小麦的 POD 活性的数量性状位点（QTL）。在染色体 3AL、4BS 和 5AS 上鉴定到三个新的 POD 活性 QTL，QPod.caas-3AL、QPod.caas-4BS 和 QPod.caas-5AS，分别解释了环境间 5.3-21.2%的表型变异。通过同源克隆和 PCR 验证，对染色体 3A 上的一个 POD 基因，命名为 TaPod-A1，进行了全长基因组 DNA（gDNA）序列的特征描述。基于 TaPod-A1 基因座两个等位基因之间的单核苷酸多态性（SNP），开发了两个互补的显性序列标签位点（STS）标记 POD-3A1 和 POD-3A2，分别在 POD 活性较低和较高的品种中扩增 291-和 766-bp 片段。使用中国春（CS）单体型缺失四体系、3AL 和 3AS 双端体系，在一套染色体 3AL 上对这两个基因特异性标记进行了作图。QTL 分析表明，QPod.caas-3AL 与基因特异性标记 POD-3A1 和 POD-3A2 共分离。在 281 个小麦品种和品系上验证了 POD-3A1 和 POD-3A2，它们与 POD 活性显著相关（P < 0.05）。POD-3A1 和 POD-3A2 可作为提高小麦育种计划中颜色属性的有用标记。
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