Lima Manoel J A, Fernandes Ridvan N, Tanaka Auro A, Reis Boaventura F
Departamento de Química, Universidade Federal do Maranhão, São Luís, Brazil.
Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, Brazil.
Luminescence. 2016 Feb;31(1):288-94. doi: 10.1002/bio.2960. Epub 2015 Jul 2.
This paper describes a new technique for the determination of captopril in pharmaceutical formulations, implemented by employing multicommuted flow analysis. The analytical procedure was based on the reaction between hypochlorite and captopril. The remaining hypochlorite oxidized luminol that generated electromagnetic radiation detected using a homemade luminometer. To the best of our knowledge, this is the first time that this reaction has been exploited for the determination of captopril in pharmaceutical products, offering a clean analytical procedure with minimal reagent usage. The effectiveness of the proposed procedure was confirmed by analyzing a set of pharmaceutical formulations. Application of the paired t-test showed that there was no significant difference between the data sets at a 95% confidence level. The useful features of the new analytical procedure included a linear response for captopril concentrations in the range 20.0-150.0 µmol/L (r = 0.997), a limit of detection (3σ) of 2.0 µmol/L, a sample throughput of 164 determinations per hour, reagent consumption of 9 µg luminol and 42 µg hypochlorite per determination and generation of 0.63 mL of waste. A relative standard deviation of 1% (n = 6) for a standard solution containing 80 µmol/L captopril was also obtained.
本文介绍了一种采用多通道流动分析技术测定药物制剂中卡托普利含量的新方法。该分析方法基于次氯酸盐与卡托普利之间的反应。剩余的次氯酸盐氧化鲁米诺，产生的电磁辐射用自制的发光计进行检测。据我们所知，这是该反应首次用于测定药品中的卡托普利，提供了一种清洁的分析方法，试剂用量最少。通过分析一组药物制剂证实了所提方法的有效性。配对t检验的应用表明，在95%置信水平下，数据集之间没有显著差异。新分析方法的有用特性包括卡托普利浓度在20.0 - 150.0 µmol/L范围内呈线性响应（r = 0.997），检测限（3σ）为2.0 µmol/L，每小时样品通量为164次测定，每次测定鲁米诺试剂消耗量为9 µg，次氯酸盐消耗量为42 µg，产生0.63 mL废液。对于含80 µmol/L卡托普利的标准溶液，相对标准偏差为1%（n = 6）。
