使用¹¹C-乙酸微PET成像对小鼠心肌血流和梗死面积进行重测的重复性
Test-retest repeatability of myocardial blood flow and infarct size using ¹¹C-acetate micro-PET imaging in mice.
作者信息
Croteau Etienne, Renaud Jennifer M, McDonald Matthew, Klein Ran, DaSilva Jean N, Beanlands Rob S B, deKemp Robert A
机构信息
National Cardiac PET Centre, University of Ottawa Heart Institute, 40 Ruskin St., Ottawa, Ontario, K1Y 4W7, Canada,
出版信息
Eur J Nucl Med Mol Imaging. 2015 Sep;42(10):1589-600. doi: 10.1007/s00259-015-3111-9. Epub 2015 Jul 5.
PURPOSE
Global and regional responses of absolute myocardial blood flow index (iMBF) are used as surrogate markers to assess response to therapies in coronary artery disease. In this study, we assessed the test-retest repeatability of iMBF imaging, and the accuracy of infarct sizing in mice using (11)C-acetate PET.
METHODS
(11)C-Acetate cardiac PET images were acquired in healthy controls, endothelial nitric oxide synthase (eNOS) knockout transgenic mice, and mice after myocardial infarction (MI) to estimate global and regional iMBF, and myocardial infarct size compared to (18)F-FDG PET and ex-vivo histology results.
RESULTS
Global test-retest iMBF values had good coefficients of repeatability (CR) in healthy mice, eNOS knockout mice and normally perfused regions in MI mice (CR = 1.6, 2.0 and 1.5 mL/min/g, respectively). Infarct size measured on (11)C-acetate iMBF images was also repeatable (CR = 17 %) and showed a good correlation with the infarct sizes found on (18)F-FDG PET and histopathology (r (2) > 0.77; p < 0.05).
CONCLUSION
(11)C-Acetate micro-PET assessment of iMBF and infarct size is repeatable and suitable for serial investigation of coronary artery disease progression and therapy.
目的
绝对心肌血流指数（iMBF）的整体和区域反应被用作评估冠状动脉疾病治疗反应的替代标志物。在本研究中，我们评估了iMBF成像的重测重复性，以及使用（11）C-乙酸PET对小鼠梗死面积测量的准确性。
方法
在健康对照、内皮型一氧化氮合酶（eNOS）基因敲除转基因小鼠和心肌梗死（MI）后的小鼠中采集（11）C-乙酸心脏PET图像，以估计整体和区域iMBF，并将心肌梗死面积与（18）F-FDG PET和离体组织学结果进行比较。
结果
在健康小鼠、eNOS基因敲除小鼠和MI小鼠的正常灌注区域，整体重测iMBF值具有良好的重复性系数（CR）（分别为CR = 1.6、2.0和1.5 mL/min/g）。（11）C-乙酸iMBF图像上测量的梗死面积也具有重复性（CR = 17%），并且与（18）F-FDG PET和组织病理学上发现的梗死面积具有良好的相关性（r（2）> 0.77；p < 0.05）。
结论
（11）C-乙酸微型PET对iMBF和梗死面积的评估具有重复性，适用于冠状动脉疾病进展和治疗的系列研究。
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