Driguez Patrick, Li Yuesheng, Gaze Soraya, Pearson Mark S, Nakajima Rie, Trieu Angela, Doolan Denise L, Felgner Philip L, Hou Xunya, Cardoso Fernanda C, Jasinskas Algis, Gobert Geoffrey N, Loukas Alex, McManus Donald P
QIMR Berghofer Medical Research Institute, Brisbane.
QIMR Berghofer Medical Research Institute, Brisbane Hunan Institute of Parasitic Diseases, Yueyang, China.
J Infect Dis. 2016 Jan 1;213(1):122-30. doi: 10.1093/infdis/jiv356. Epub 2015 Jul 6.
Infection with Schistosoma japonicum causes high levels of pathology that is predominantly determined by the cellular and humoral response of the host. However, the specific antibody response that arises during the development of disease is largely undescribed in Asian schistosomiasis-endemic populations. A schistosome protein microarray was used to compare the antibody profiles of subjects with acute infection, with early or advanced disease associated with severe pathology, with chronic infection, and subjects exposed but stool negative for S. japonicum eggs to the antibody profiles of nonexposed controls. Twenty-five immunodominant antigens were identified, including vaccine candidates, tetraspanin-related proteins, transporter molecules, and unannotated proteins. Additionally, individuals with severe pathology had a limited specific antibody response, suggesting that individuals with mild disease may use a broad and strong antibody response, particularly against surface-exposed proteins, to control pathology and/or infection. Our study has identified specific antigens that can discriminate between S. japonicum-exposed groups with different pathologies and may also allow the host to control disease pathology and provide resistance to parasite infection.
日本血吸虫感染会引发严重的病理变化，这种病理变化主要由宿主的细胞和体液反应决定。然而，在亚洲血吸虫病流行地区的人群中，疾病发展过程中产生的特异性抗体反应在很大程度上尚未得到描述。利用血吸虫蛋白微阵列比较了急性感染患者、与严重病理相关的早期或晚期疾病患者、慢性感染患者以及接触过血吸虫但粪便日本血吸虫卵检测呈阴性的受试者与未接触过血吸虫的对照者的抗体谱。鉴定出了25种免疫显性抗原，包括候选疫苗、四跨膜蛋白相关蛋白、转运分子和未注释的蛋白。此外，有严重病理变化的个体特异性抗体反应有限，这表明病情较轻的个体可能会利用广泛而强烈的抗体反应，尤其是针对表面暴露蛋白的反应，来控制病理变化和/或感染。我们的研究确定了能够区分具有不同病理变化的日本血吸虫暴露组的特异性抗原，这些抗原也可能使宿主控制疾病病理变化并对寄生虫感染产生抵抗力。
