Li Yanmeng, Zhao Ruike, Zhang Xianfeng, Han Qingzhen, Qian Xuefeng, Gu Guohao, Shi Jinfang, Xu Jie
Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University , Suzhou, P.R. of China .
Faculty, Department of Clinical Laboratory, the First Affiliated Hospital of Soochow University , Suzhou,P.R. of China .
J Clin Diagn Res. 2015 May;9(5):DC11-4. doi: 10.7860/JCDR/2015/12090.5990. Epub 2015 May 1.
Methicillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen that causes a variety of infections. MRSA has evolved resistance to multiple antibiotics. Genetic background and virulence differs in different geographic regions. The present study was aimed to investigate the prevalence of enterotoxin genes and spa genotypes of hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) isolated from a tertiary care hospital of Jiangsu province, China.
HA-MRSA isolates from August 2013 to April 2014 at a tertiary care hospital of China were collected. We investigated antimicrobial pattern, spa types, SCCmec types and the presence of 14 virulence genes.
Eighty HA-MRSA isolates were collected. Results from SCCmec typing revealed that 73.8% were type II; 13.8% were type III; 12.5% were type V. There were 19 different spa types. Spa type t2460 was the most common (35.0%), followed by t002 (11.3%). CC5 was the predominant MLST CCs type (50%). The most frequent toxin genes were sea, seb, sed, sel, sen and seo (100.0%). None of the investigated isolates carried the sec or tst.
Genotypic and virulence evaluation of the isolated HA-MRSA revealed that the isolates with CC5 and SCCmec II were the predominant type and highly homological. The virulence profiles mainly existed in the genes of sea, seb, sed, sel, sen, seo and ser. The prevalence of t2460 was an outbreak and the predominant spa type.
耐甲氧西林金黄色葡萄球菌（MRSA）是一种主要的医院病原体，可引起多种感染。MRSA已对多种抗生素产生耐药性。不同地理区域的遗传背景和毒力有所不同。本研究旨在调查从中国江苏省一家三级医院分离出的医院相关耐甲氧西林金黄色葡萄球菌（HA-MRSA）的肠毒素基因流行情况和spa基因型。
收集了2013年8月至2014年4月在中国一家三级医院分离出的HA-MRSA菌株。我们调查了抗菌模式、spa类型、SCCmec类型以及14种毒力基因的存在情况。
共收集到80株HA-MRSA菌株。SCCmec分型结果显示，73.8%为II型；13.8%为III型；12.5%为V型。有19种不同的spa类型。spa类型t2460最为常见（35.0%），其次是t002（11.3%）。CC5是主要的多位点序列分型（MLST）CCs类型（50%）。最常见的毒素基因是sea、seb、sed、sel、sen和seo（100.0%）。所调查的菌株均未携带sec或tst。
对分离出的HA-MRSA进行的基因型和毒力评估显示，CC5和SCCmec II型菌株是主要类型且高度同源。毒力谱主要存在于sea、seb、sed、sel、sen、seo和ser基因中。t2460的流行是一次暴发且是主要的spa类型。
