Penniyaynen V A, Kipenko A V, Lopatina E V, Bagrov A Ya, Krylov B V
Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, 199034, Russia.
Dokl Biol Sci. 2015;462:164-6. doi: 10.1134/S0012496615030096. Epub 2015 Jul 12.
In recent years, a substantial interest has been aroused in investigating Na(+),K(+) ATPase as a membrane structure which not only performs its direct function in maintaining the gradients of Na(+) and K(+) concentrations, but also may participate in the intracellular signal transduction processes in response to various physiological stimuli. The effect of marinobufagenin, a digitalislike factor, on the growth and proliferation of sensory ganglia, cardiac, retina, skin, and liver tissue explants of 10-12day old chicken embryos was investigated by the organotypic culture method in a wide range of concentrations (10(-10) to 10(-4) M). It was first demonstrated that marinobufagenin inhibited the growth of the investigated tissue explants in the dosedependent but not tissuespecific manner. The experimental data obtained allow us to suggest that the mechanism of regulation of tissue growth by marinobufagenin during the embryonic period of ontogenesis is associated with the modulation of the pump function of Na(+),K(+) ATPase but not its transducing function.
近年来，人们对研究钠钾ATP酶产生了浓厚兴趣，它作为一种膜结构，不仅在维持钠钾浓度梯度方面发挥直接作用，还可能参与细胞内信号转导过程以响应各种生理刺激。通过器官型培养方法，在广泛的浓度范围（10⁻¹⁰至10⁻⁴ M）内研究了洋地黄样因子海蟾蜍精对10 - 12日龄鸡胚感觉神经节、心脏、视网膜、皮肤和肝脏组织外植体生长和增殖的影响。首次证明海蟾蜍精以剂量依赖性而非组织特异性方式抑制所研究组织外植体的生长。所获得的实验数据使我们能够推测，在个体发育的胚胎期，海蟾蜍精调节组织生长的机制与钠钾ATP酶泵功能的调节有关，而非其转导功能。
