[哇巴因对器官型培养中细胞生长和增殖的影响]

[The influence of ouabagenin on the growth and proliferation of cells in the organotypical culture].

作者信息

Penniiaĭnen V A, Kipenko A V, Lopatina E V, Krylov B V

出版信息

Ross Fiziol Zh Im I M Sechenova. 2014 Nov;100(11):1303-9.

Abstract

The aim of the present work was to investigate the effect of ouabagenin on the growth and proliferation of cells in organotypic culture. The objects of study were explants of nerve, cardiac, retina and liver tissue of 10-12 day old chicken embryos. Inhibitor of Na+,K(+)-ATPase ouabagenin was investigated in a wide range of concentrations (0.1 nM-1 mM). It has been found that the ouabagenin controls cell growth and proliferation in a dose-dependent manner and tissue-unspecific. The data obtained show that ouabagenin regulates only the pumping function of Na+,K(+)-ATPase.

摘要

本研究的目的是探讨哇巴因对器官型培养中细胞生长和增殖的影响。研究对象是10 - 12日龄鸡胚的神经、心脏、视网膜和肝脏组织外植体。对Na +,K(+)-ATP酶抑制剂哇巴因在广泛的浓度范围（0.1 nM - 1 mM）内进行了研究。已发现哇巴因以剂量依赖性方式且不具有组织特异性地控制细胞生长和增殖。所获得的数据表明，哇巴因仅调节Na +,K(+)-ATP酶的泵功能。