Cho Jaeeun, Lim Hyemi, Jung Bong-Kwang, Shin Eun-Hee, Chai Jong-Yil
Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea ; Tropical Medicine, Seoul National University Bundang Hospital, Seongnam 463-707, Korea.
Korean J Parasitol. 2015 Jun;53(3):349-53. doi: 10.3347/kjp.2015.53.3.349. Epub 2015 Jun 30.
Anisakis simplex sensu stricto (s.s.), Anisakis pegreffii, Anisakis berlandi (=A. simplex sp. C), and Anisakis typica are the 4 major species of Anisakis type I larvae. In the Republic of Korea (Korea), A. pegreffii, A. berlandi, and A. typica larvae in fish hosts has seldom been documented. In this study, molecular analysis was performed on Anisakis larvae from the sea eels (Astroconger myriaster), the major source of human anisakiasis in Korea, collected from Tongyeong City, a southern coastal area of Korea. All 20 sea eels examined were infected with Anisakis type I larvae (160 larvae; 8 per fish). Their species were analyzed using PCR-RFLP patterns and nucleotide sequences of internal transcribed spacers (ITS1, 5.8 subunit gene, and ITS2) and mitochondrial cytochrome c oxidase 2 (cox2). Most (86.8%; 112/129) of the Anisakis type I larvae were A. pegreffii, and 7.8% (10/129) were A. typica. The remaining 5.4% (7/129) was not identified. Thus, A. pegreffii is the major species of anisakid larvae in sea eels of the southern coast of Korea.
简单异尖线虫（狭义）、佩氏异尖线虫、贝氏异尖线虫（=简单异尖线虫C型）和典型异尖线虫是异尖线虫I型幼虫的4个主要种类。在大韩民国（韩国），鱼类宿主中的佩氏异尖线虫、贝氏异尖线虫和典型异尖线虫幼虫鲜有记录。在本研究中，对从韩国南部沿海地区统营市采集的海鳗（星康吉鳗）中的异尖线虫幼虫进行了分子分析，海鳗是韩国人类异尖线虫病的主要来源。所检查的20条海鳗均感染了异尖线虫I型幼虫（共160条幼虫；每条鱼8条）。利用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）模式以及内部转录间隔区（ITS1、5.8亚基基因和ITS2）和线粒体细胞色素c氧化酶2（cox2）的核苷酸序列对其种类进行了分析。大多数（86.8%；112/129）异尖线虫I型幼虫为佩氏异尖线虫，7.8%（10/129）为典型异尖线虫。其余5.4%（7/129）未鉴定出来。因此，佩氏异尖线虫是韩国南部沿海海鳗中异尖线虫幼虫的主要种类。
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