Process & Analytical Chemistry, Merck Research Laboratories, Mailstop: 800-D133, 126 E. Scott Ave, Rahway, NJ 07065 (USA).
Bruker Biospin GmbH, Silberstreifen, 76287 Rheinstetten (Germany).
Angew Chem Int Ed Engl. 2015 Aug 24;54(35):10160-4. doi: 10.1002/anie.201502540. Epub 2015 Jul 15.
Cryptospirolepine is the most structurally complex alkaloid discovered and characterized thus far from any Cryptolepis specie. Characterization of several degradants of the original, sealed NMR sample a decade after the initial report called the validity of the originally proposed structure in question. We now report the development of improved, homodecoupled variants of the 1,1- and 1,n-ADEQUATE (HD-ADEQUATE) NMR experiments; utilization of these techniques was critical to successfully resolving long-standing structural questions associated with crytospirolepine.
Cryptospirolepine 是迄今为止从任何一种 Cryptolepis 物种中发现和鉴定的结构最复杂的生物碱。在最初报告的十年后，对原始密封 NMR 样品的几种降解产物的鉴定，使最初提出的结构的有效性受到质疑。我们现在报告了 1,1- 和 1,n-ADEQUATE（HD-ADEQUATE）NMR 实验的改进、同核去耦变体的开发；这些技术的利用对于成功解决与 crytospirolepine 相关的长期存在的结构问题至关重要。
