Mahmoud Y I, Mahmoud A A, Nassar G
a Zoology Department, Faculty of Science , Ain Shams University , Cairo , Egypt.
Biotech Histochem. 2015;90(8):594-600. doi: 10.3109/10520295.2015.1063005. Epub 2015 Jul 16.
Acetaminophen (paracetamol) is a well-tolerated analgesic and antipyretic drug when used at therapeutic doses. Overdoses, however, cause oxidative stress, which leads to acute liver failure. Alpha lipoic acid is an antioxidant that has proven effective for ameliorating many pathological conditions caused by oxidative stress. We evaluated the effect of alpha lipoic acid on the histological and histochemical alterations of liver caused by an acute overdose of acetaminophen in rats. Livers of acetaminophen-intoxicated rats were congested and showed centrilobular necrosis, vacuolar degeneration and inflammatory cell infiltration. Necrotic hepatocytes lost most of their carbohydrates, lipids and structural proteins. Liver sections from rats pre-treated with lipoic acid showed fewer pathological changes; the hepatocytes appeared moderately vacuolated with moderate staining of carbohydrates and proteins. Nevertheless, alpha lipoic acid at the dose we used did not protect the liver fully from acetaminophen-induced acute toxicity.
对乙酰氨基酚（扑热息痛）在治疗剂量使用时是一种耐受性良好的镇痛和解热药物。然而，过量服用会导致氧化应激，进而引发急性肝衰竭。α硫辛酸是一种抗氧化剂，已被证明对改善许多由氧化应激引起的病理状况有效。我们评估了α硫辛酸对大鼠急性过量服用对乙酰氨基酚所致肝脏组织学和组织化学改变的影响。对乙酰氨基酚中毒大鼠的肝脏充血，呈现中央小叶坏死、空泡变性和炎症细胞浸润。坏死的肝细胞失去了大部分碳水化合物、脂质和结构蛋白。用硫辛酸预处理的大鼠肝脏切片显示病理变化较少；肝细胞出现中度空泡化，碳水化合物和蛋白质染色适中。然而，我们使用的剂量的α硫辛酸并不能完全保护肝脏免受对乙酰氨基酚诱导的急性毒性。
