Rodrigues Francisca, Santos Joana, Pimentel Filipa B, Braga Nair, Palmeira-de-Oliveira Ana, Oliveira M Beatriz P P
REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
Food Funct. 2015 Aug;6(8):2854-60. doi: 10.1039/c5fo00571j. Epub 2015 Jul 20.
The present study was aimed to assess the macronutrient composition and the amino acid and vitamin E profiles of Castanea sativa shell from different production regions of Portugal (Minho, Trás-os-Montes and Beira-Alta). The nutritional composition was similar for all samples, with a high moisture content and low fat amounts. Arginine and leucine were the predominant essential amino acids (EAA) accounting for 3.55-7.21% and 1.59-2.08%, respectively, for samples of the different production zones. All the shells presented high contents of vitamin E (481.5 mg per 100 g sample, 962.8 mg per 100 g sample and 567.5 mg per 100 g sample, respectively, for Minho, Trás-os-Montes and Beira-Alta). The predominant vitamer was γ-tocopherol (670 mg per 100 g sample for Trás-os-Montes). The antimicrobial and antioxidant activities of C. sativa shell were also determined. Trás-os-Montes extracts displayed the highest antioxidant activity (EC50 = 31.8 ± 1.3 μg mL(-1) for DPPH; 8083.5 ± 164.8 μmol per mg db for FRAP). The total phenolic content (TPC) varied from 241.9 mg to 796.8 mg gallic acid equivalents (GAE) per g db sample, the highest TPC being obtained for Trás-os-Montes. The total flavonoid content (TFC) varied from 31.4 to 43.3 mg of catechin equivalents (CEQ) per g db sample. No antimicrobial activity was observed. The results showed the potentialities of C. sativa shell extracts.
本研究旨在评估来自葡萄牙不同产区（米尼奥、特拉斯-奥斯-蒙特斯和贝拉-阿尔塔）的欧洲栗壳的常量营养素组成、氨基酸和维生素E谱。所有样品的营养成分相似，水分含量高，脂肪含量低。精氨酸和亮氨酸是主要的必需氨基酸（EAA），不同产区样品中分别占3.55 - 7.21%和1.59 - 2.08%。所有栗壳的维生素E含量都很高（米尼奥、特拉斯-奥斯-蒙特斯和贝拉-阿尔塔的样品分别为每100克样品含481.5毫克、962.8毫克和567.5毫克）。主要的维生素形式是γ-生育酚（特拉斯-奥斯-蒙特斯的样品每100克含670毫克）。还测定了欧洲栗壳的抗菌和抗氧化活性。特拉斯-奥斯-蒙特斯的提取物表现出最高的抗氧化活性（DPPH法的EC50 = 31.8 ± 1.3微克/毫升；FRAP法为每毫克干重8083.5 ± 164.8微摩尔）。总酚含量（TPC）在每克干重样品241.9毫克至796.8毫克没食子酸当量（GAE）之间变化，特拉斯-奥斯-蒙特斯的TPC最高。总黄酮含量（TFC）在每克干重样品31.4至43.3毫克儿茶素当量（CEQ）之间变化。未观察到抗菌活性。结果显示了欧洲栗壳提取物的潜力。
