Dorosh Olena, Moreira Manuela M, Pinto Diana, F Peixoto Andreia, Freire Cristina, Costa Paulo, Rodrigues Francisca, Delerue-Matos Cristina
REQUIMTE/LAQV, Instituto Superior de Engenharia do Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida, 431, 4249-015 Porto, Portugal.
REQUIMTE/LAQV, Departamento. de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal.
Foods. 2020 Jul 3;9(7):872. doi: 10.3390/foods9070872.
This work focused on evaluating the possibility of using vineyard pruning wastes from two Portuguese varieties; Touriga Nacional (TN) and Tinta Roriz (TR), as new potential ingredients for the nutraceutical industry. An environmentally friendly extraction technique; namely subcritical-water extraction (SWE), was employed. The overall results indicate that phenolic acids were the major class of compounds quantified; being gallic acid the principal one. The highest value for total phenolic content (TPC) was obtained for the TR extract at 250 °C (181 ± 12 mg GAE/g dw). In terms of antioxidant activity; the DPPH values for the extracts obtained at 250 °C were approximately 4-fold higher than the ones obtained at 125 °C; with TR extract presenting the highest value (203 ± 22 mg TE/g dw). Thus, the TR extract obtained through SWE at 250 °C was selected to evaluate the scavenging activity and the in vitro effects on cells due to the best results achieved in the previous assays. This extract presented the ability to scavenge reactive oxygen species (O, HOCl and ROO). No adverse effects were observed in HFF-1 viability after exposure to extract concentrations below 100 μg/mL. This work demonstrated that vine-canes extracts could be a potential ingredient to nutraceutical industry.
这项工作聚焦于评估利用来自两个葡萄牙品种（国家杜丽佳（TN）和罗丽红（TR））葡萄园修剪废弃物作为营养保健品行业新潜在成分的可能性。采用了一种环境友好型提取技术，即亚临界水萃取（SWE）。总体结果表明，酚酸是定量分析的主要化合物类别，其中没食子酸是主要成分。在250℃下获得的TR提取物的总酚含量（TPC）最高（181±12毫克没食子酸当量/克干重）。就抗氧化活性而言，在250℃下获得的提取物的DPPH值比在125℃下获得的约高4倍，TR提取物的值最高（203±22毫克 Trolox当量/克干重）。因此，由于在先前试验中取得了最佳结果，选择在250℃下通过SWE获得的TR提取物来评估其清除活性和对细胞的体外作用。该提取物具有清除活性氧（O、HOCl和ROO）的能力。在暴露于浓度低于100μg/mL的提取物后，未观察到对人包皮成纤维细胞-1（HFF-1）活力有不良影响。这项工作表明，葡萄藤提取物可能成为营养保健品行业的一种潜在成分。
