Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.
Department of Research and Development, Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran.
Food Environ Virol. 2019 Jun;11(2):193-197. doi: 10.1007/s12560-019-09377-1. Epub 2019 Mar 20.
Salivirus is a newly discovered virus which seems to be related to acute gastroenteritis in children. Salivirus may infect susceptible children by fecal-oral route after exposure to contaminated water. The present study aims to evaluate the occurrence and quantity of Salivirus in treated and untreated sewage water and river water samples collected in the city of Karaj, Iran by reverse transcription-quantitative PCR assay. A total of 50 samples were collected from environmental waters containing 22 treated and untreated sewage water in volume of 1 l and 28 river water samples in volume of 5 l were included in this study. After viral RNA extraction, the Real-time PCR was performed to amplify the 5'UTR sequence of Salivirus genome and viral load was assessed. Out of the 50 samples tested, the Salivirus genomic RNA was identified in 5/12 (41.6%) of treated and 3/10 (30%) of untreated sewage samples and in 8/28 (28.5%) of river water samples. The maximum viral load was 4.8 × 10 copies/l in treated sewage water sample in September and the lower viral load was 4 × 10 copies/l related to treated sewage water taken in December. This is the first report of Salivirus occurrence in the environmental waters in Iran. The viral prevalence of Salivirus in each of the three sets of tested samples was within low to moderate in range.
唾液病毒是一种新发现的病毒，似乎与儿童急性肠胃炎有关。唾液病毒可能通过粪-口途径在接触受污染的水后感染易感儿童。本研究旨在通过逆转录定量 PCR 检测评估伊朗卡拉季市处理和未处理污水和河水样本中唾液病毒的发生和数量。共采集 50 份环境水样，包括 22 份 1 升处理和未处理污水和 28 份 5 升河水。在提取病毒 RNA 后，进行实时 PCR 扩增唾液病毒基因组的 5'UTR 序列，并评估病毒载量。在检测的 50 个样本中，在 12 个处理污水样本中的 5 个（41.6%）和 10 个未处理污水样本中的 3 个（30%）以及 28 个河水样本中的 8 个（28.5%）中检测到唾液病毒基因组 RNA。9 月处理污水样本中的最大病毒载量为 4.8×10 拷贝/升，12 月处理污水样本中的最低病毒载量为 4×10 拷贝/升。这是伊朗环境水中唾液病毒首次被发现。在三组检测样本中，唾液病毒的病毒流行率均处于低到中度范围内。
