Wang Yang, Guo Rui, Hou Xufeng, Lei Mengmeng, Zhou Qihang, Xu Zhihong
Key Laboratory of Chemo/Biosensing and Detection, School of Chemistry and Chemical Engineering, Xuchang University, Xuchang, 461000, People's Republic of China.
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, 450001, People's Republic of China.
J Fluoresc. 2019 May;29(3):645-652. doi: 10.1007/s10895-019-02378-0. Epub 2019 Apr 26.
A simple rhodamine-based fluorescent probe L1, which exhibits good response to Fe in CHCN/Tris-HCl buffer (v:v = 9:1, pH 7.00) solution. With the experimental conditions optimized, the probe L1 could be used as a fluorescent and colorimetric probe for Fe with the detection limit as low as 0.29 μM. The binding constant (K) of Fe binding to the probe L1 were calculated to be 1.4 × 10 M, respectively from a Benesi-Hildebrand plot. With the addition of Fe, the CHCN/Tris-HCl buffer (v:v = 9:1, pH 7.00) solution of the probe L1 exhibited a obviously color change from transparent to pink with distinctive changes. More importantly, the recognition process of the probe L1 for Fe was chemically reversible on the addition AcO. Graphical Abstract.
一种基于罗丹明的简单荧光探针L1，在CHCN/Tris-HCl缓冲液（体积比v:v = 9:1，pH 7.00）溶液中对Fe表现出良好的响应。在优化实验条件下，探针L1可用作Fe的荧光和比色探针，检测限低至0.29 μM。根据Benesi-Hildebrand图计算得出Fe与探针L1的结合常数（K）分别为1.4×10 M。加入Fe后，探针L1的CHCN/Tris-HCl缓冲液（体积比v:v = 9:1，pH 7.00）溶液呈现出明显的颜色变化，从透明变为粉红色，变化显著。更重要的是，在加入AcO时，探针L1对Fe的识别过程在化学上是可逆的。图形摘要。
