School of Nutrition, Chung Shan Medical University, Taichung City, Taiwan.
Department of Nutrition, Chung Shan Medical University Hospital, Taichung City, Taiwan.
Eur J Nutr. 2016 Mar;55(2):845-854. doi: 10.1007/s00394-015-0994-y. Epub 2015 Jul 22.
The anti-glycative and anti-oxidative effects from Houttuynia cordata leaves aqueous extract (HCAE) in heart and kidney of diabetic mice were examined.
HCAE, at 1 or 2 %, was supplied in drinking water for 8 weeks. Plasma glucose and blood urea nitrogen (BUN) levels and creatine phosphokinase (CPK) activity were measured. The production of oxidative and inflammatory factors was determined. Activity and protein expression of associated enzymes or regulators were analyzed.
HCAE intake at both doses lowered plasma glucose and BUN levels, and CPK activity and also restored creatinine clearance rate in diabetic mice. HCAE intake, only at 2 %, retained plasma insulin levels (P < 0.05). HCAE reduced reactive oxygen species, protein carbonyl, interleukin-6, tumor necrosis factor-alpha, N (ε) -(carboxymethyl)-lysine, pentosidine and fructose levels, and reserved glutathione content in heart and kidney of diabetic mice (P < 0.05). Diabetes enhanced aldose reductase (AR) activity and protein expression in heart and kidney (P < 0.05). HCAE intake at both doses decreased renal AR activity and protein expression, but only at 2 % lowered cardiac AR activity and protein expression (P < 0.05). Diabetes increased protein expression of RAGE, p47(phox) and gp91(phox), nuclear factor kappa-B (NF-κB) p50, NF-κB p65 and mitogen-activated protein kinase in heart and kidney (P < 0.05). HCAE intake only at 2 % limited RAGE expression, but at 1 and 2 % downregulated p47(phox), NF-κB p65 and p-p38 expression in these organs (P < 0.05).
These findings suggest that Houttuynia cordata leaves aqueous extract could ameliorate cardiac and renal injury under diabetic condition.
研究鱼腥草叶水提物（HCAE）对糖尿病小鼠心脏和肾脏的抗糖化和抗氧化作用。
8 周内，糖尿病小鼠通过饮用水摄入 HCAE（1%或 2%）。测量血浆葡萄糖和血尿素氮（BUN）水平以及肌酸磷酸激酶（CPK）活性。测定氧化和炎症因子的产生。分析相关酶或调节剂的活性和蛋白表达。
两种剂量的 HCAE 摄入均降低了糖尿病小鼠的血浆葡萄糖和 BUN 水平以及 CPK 活性，并恢复了肌酐清除率。HCAE 摄入（仅 2%）可维持血浆胰岛素水平（P<0.05）。HCAE 降低了心脏和肾脏中活性氧、蛋白羰基、白细胞介素-6、肿瘤坏死因子-α、N（ε）-（羧甲基）赖氨酸、戊糖和果糖水平，并保留了谷胱甘肽含量（P<0.05）。糖尿病增强了心脏和肾脏中醛糖还原酶（AR）的活性和蛋白表达（P<0.05）。两种剂量的 HCAE 摄入均降低了肾脏 AR 的活性和蛋白表达，但仅 2%降低了心脏 AR 的活性和蛋白表达（P<0.05）。糖尿病增加了心脏和肾脏中 RAGE、p47（phox）和 gp91（phox）、核因子 kappa-B（NF-κB）p50、NF-κB p65 和丝裂原活化蛋白激酶的蛋白表达（P<0.05）。HCAE 摄入（仅 2%）仅限制了 RAGE 的表达，但在这些器官中，1%和 2%的 HCAE 降低了 p47（phox）、NF-κB p65 和 p-p38 的表达（P<0.05）。
这些发现表明，鱼腥草叶水提物可改善糖尿病条件下的心脏和肾脏损伤。
