Kim Gi Dae
Department of Food and Nutrition, Kyungnam University, Gyeongnam 51767, Korea.
Prev Nutr Food Sci. 2020 Mar 31;25(1):108-112. doi: 10.3746/pnf.2020.25.1.108.
(HC) is a herb widely used in traditional Asian medicine as an ingredient in complex prescriptions. HC is known for its anti-leukemic, anti-oxidative, and anti-inflammatory properties. However, its anti-vascular endothelial aging efficacy and the underlying mechanisms are not fully understood. In this study, we investigated the anti-aging effects of HC in a high glucose (HG)-induced endothelial cell (EC)-aging model. Treatment with HC (40 μg/mL) increased migration of ECs, and increased phosphorylation of extracellular signal-regulated kinases and p38 in a dose-dependent manner. Following HG treatment (30 mM), HC significantly decreased the number of senescence-associated β-galactosidase positive cells, which are the biomarkers for aging, in a dose-dependent manner. Based on levels of phosphorylation, HC (40 μg/mL) was shown to increase expression of sirtuin1 (Sirt1) and endothelial nitric oxide synthase (eNOS) by 74.4% and 328.2%, respectively. Furthermore, treatment of HG-induced senescent ECs with HC (40 μg/mL) significantly increased nitric oxide production (<0.05). These results demonstrate that HC both increases EC migration and regulates the Sirt1/eNOS pathway, suggesting HC has potential for protecting ECs against HG-induced aging.
(HC) 是一种在传统亚洲医学中作为复方制剂成分被广泛使用的草药。HC 以其抗白血病、抗氧化和抗炎特性而闻名。然而，其抗血管内皮衰老的功效及潜在机制尚未完全明确。在本研究中，我们在高糖 (HG) 诱导的内皮细胞 (EC) 衰老模型中研究了 HC 的抗衰老作用。用 HC（40 μg/mL）处理可增加 EC 的迁移，并以剂量依赖方式增加细胞外信号调节激酶和 p38 的磷酸化。在 HG 处理（30 mM）后，HC 以剂量依赖方式显著降低衰老相关β-半乳糖苷酶阳性细胞的数量，这些细胞是衰老的生物标志物。基于磷酸化水平，HC（40 μg/mL）显示可使沉默信息调节因子 1（Sirt1）和内皮型一氧化氮合酶（eNOS）的表达分别增加 74.4% 和 328.2%。此外，用 HC（40 μg/mL）处理 HG 诱导的衰老 EC 可显著增加一氧化氮的产生（<0.05）。这些结果表明，HC 既能增加 EC 迁移又能调节 Sirt1/eNOS 途径，提示 HC 具有保护 EC 免受 HG 诱导衰老的潜力。
