Cetin Damla, Hacımuftuoglu Ahmet, Tatar Abdulgani, Turkez Hasan, Togar Basak
Department of Medical Pharmacology, Faculty of Medicine, Kafkas University, Kars, Turkey.
Department of Medical Pharmacology, Faculty of Medicine, Atatürk University, Erzurum, Turkey.
Cytotechnology. 2016 Aug;68(4):1361-7. doi: 10.1007/s10616-015-9896-3. Epub 2015 Jul 22.
Paclitaxel (PAC) and cisplatin (CIS) are two established chemotherapeutic drugs used in combination for the treatment of various solid tumors. However, the usage of PAC and CIS are limited because of the incidence of their moderate or severe neurotoxic side effects. In this study, we aimed to assess the protective role of salicylic acid (SA) against neurotoxicity caused by PAC and CIS. For this purpose, newborn Sprague Dawley rats were decapitated in sterile atmosphere and primary cortex neuron cultures were established. On the 10th day SA was added into culture plates. PAC and CIS were added on the 12th day. The cytotoxicity was determined by using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Oxidative alterations were assessed using total antioxidant capacity and total oxidative stress assays in rat primary neuron cell cultures. It was shown that both concentrations of PAC and CIS treatments caused neurotoxicity. Although SA decreased the neurotoxicity by CIS and PAC, it was more effective against the toxicity caused by CIS rather than the toxicity caused by PAC. In conclusion it was clearly revealed that SA decreased the neurotoxic effect of CIS and PAC in vitro.
紫杉醇（PAC）和顺铂（CIS）是两种已确立的化疗药物，联合用于治疗各种实体瘤。然而，由于它们中度或重度神经毒性副作用的发生率，PAC和CIS的使用受到限制。在本研究中，我们旨在评估水杨酸（SA）对PAC和CIS所致神经毒性的保护作用。为此，在无菌环境下断头新生Sprague Dawley大鼠，建立原代皮层神经元培养物。在第10天，将SA加入培养板中。在第12天加入PAC和CIS。使用MTT [3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐] 测定法测定细胞毒性。在大鼠原代神经元细胞培养物中，使用总抗氧化能力和总氧化应激测定法评估氧化改变。结果表明，PAC和CIS两种浓度的处理均引起神经毒性。虽然SA降低了CIS和PAC所致的神经毒性，但它对CIS所致毒性的作用比对PAC所致毒性的作用更有效。总之，清楚地表明SA在体外降低了CIS和PAC的神经毒性作用。
