Vaitheeswari S, Sriram R, Brindha P, Kurian Gino A
School of Chemical and Biotechnology, SASTRA University, Thanjavur, T. N, India.
Vascular Biology Lab, SASTRA University, Thanjavur, T. N, India.
Int Braz J Urol. 2015 May-Jun;41(3):503-10. doi: 10.1590/S1677-5538.IBJU.2014.0193.
Calcium oxalate urolithiasis is one of the most common urinary tract diseases and is of high prevalence. The present study proposes to evaluate the antilithiatic property of hydrogen sulfide and its metabolites like thiosulfate & sulfate in an in vitro model.
The antilithiatic activity of sodium hydrogen sulfide (NaSH), sodium thiosulfate (Na(2)S(2)O(3)) and sodium sulfate (Na(2)SO(4)) on the kinetics of calcium oxalate crystal formation was investigated both in physiological buffer and in urine from normal and recurrent stone forming volunteers. The stones were characterized by optical and spectroscopic techniques.
The stones were characterized to be monoclinic, prismatic and bipyramidal habit which is of calcium monohydrate and dihydrate nature. The FTIR displayed fingerprint corresponding to calcium oxalate in the control while in NaSH treated, S=O vibrations were visible in the spectrum. The order of percentage inhibition was NaSH>Na(2)S(2)O(3)>Na(2)SO(4).
Our study indicates that sodium hydrogen sulfide and its metabolite thiosulfate are inhibitors of calcium oxalate stone agglomeration which makes them unstable both in physiological buffer and in urine. This effect is attributed to pH changes and complexing of calcium by S(2)O(3)(2)-and SO(4)(2)- moiety produced by the test compounds.
草酸钙尿路结石是最常见的泌尿系统疾病之一，发病率很高。本研究旨在评估硫化氢及其代谢产物如硫代硫酸盐和硫酸盐在体外模型中的抗结石特性。
研究了硫化钠（NaSH）、硫代硫酸钠（Na₂S₂O₃）和硫酸钠（Na₂SO₄）对正常及复发性结石形成志愿者的生理缓冲液和尿液中草酸钙晶体形成动力学的抗结石活性。通过光学和光谱技术对结石进行表征。
结石的特征为单斜、棱柱和双锥体习性，具有一水合钙和二水合钙的性质。傅里叶变换红外光谱（FTIR）显示对照组中对应草酸钙的指纹图谱，而在NaSH处理组中，光谱中可见S=O振动。抑制百分比的顺序为NaSH>Na₂S₂O₃>Na₂SO₄。
我们的研究表明，硫化氢钠及其代谢产物硫代硫酸盐是草酸钙结石聚集的抑制剂，这使得它们在生理缓冲液和尿液中都不稳定。这种作用归因于测试化合物产生的pH变化以及S₂O₃²⁻和SO₄²⁻部分对钙的络合作用。
