Liu Jun, Guo Ting, Wang Dong, Xu Jiahui, Ying Hanjie
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, People's Republic of China.
National Engineering Technique Research Center for Biotechnology, Nanjing, People's Republic of China.
Biotechnol Appl Biochem. 2016 Sep;63(5):727-733. doi: 10.1002/bab.1418. Epub 2015 Sep 14.
A mutant strain of Clostridium beijerinckii, with high tolerance to ferulic acid, was generated using atmospheric pressure glow discharge and high-throughput screening of C. beijerinckii NCIMB 8052. The mutant strain M11 produced 7.24 g/L of butanol when grown in P2 medium containing 30 g/L of glucose and 0.5 g/L of ferulic acid, which is comparable to the production from non-ferulic acid cultures (8.11 g/L of butanol). When 0.8 g/L of ferulic acid was introduced into the P2 medium, C. beijerinckii M11 grew well and produced 4.91 g/L of butanol. Both cell growth and butanol production of C. beijerinckii M11 were seriously inhibited when 0.9 g/L of ferulic acid was added into the P2 medium. Furthermore, C. beijerinckii M11 could produce 6.13 g/L of butanol using non-detoxified hemicellulosic hydrolysate from diluted sulfuric acid-treated corn fiber (SAHHC) as the carbon source. These results demonstrate that C. beijerinckii M11 has a high ferulic acid tolerance and is able to use non-detoxified SAHHC for butanol production.
利用大气压辉光放电和对拜氏梭菌NCIMB 8052进行高通量筛选，获得了一株对阿魏酸具有高耐受性的拜氏梭菌突变株。突变株M11在含有30 g/L葡萄糖和0.5 g/L阿魏酸的P2培养基中生长时，可产生7.24 g/L的丁醇，这与在不含阿魏酸的培养基中产生的丁醇量（8.11 g/L）相当。当向P2培养基中添加0.8 g/L阿魏酸时，拜氏梭菌M11生长良好，可产生4.91 g/L的丁醇。当向P2培养基中添加0.9 g/L阿魏酸时，拜氏梭菌M11的细胞生长和丁醇产生均受到严重抑制。此外，拜氏梭菌M11可以使用经稀硫酸处理的玉米纤维（SAHHC）的未解毒半纤维素水解产物作为碳源，产生6.13 g/L的丁醇。这些结果表明，拜氏梭菌M11具有高阿魏酸耐受性，并且能够使用未解毒的SAHHC生产丁醇。
