State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, China.
J Ind Microbiol Biotechnol. 2012 Mar;39(3):401-7. doi: 10.1007/s10295-011-1017-5. Epub 2011 Jul 26.
Clostridium beijerinckii mutant strain IB4, which has a high level of inhibitor tolerance, was screened by low-energy ion implantation and used for butanol fermentation from a non-detoxified hemicellulosic hydrolysate of corn fiber treated with dilute sulfuric acid (SAHHC). Evaluation of toxicity showed C. beijerinckii IB4 had a higher level of tolerance than parent strain C. beijerinckii NCIMB 8052 for five out of six phenolic compounds tested (the exception was vanillin). Using glucose as carbon source, C. beijerinckii IB4 produced 9.1 g l(-1) of butanol with an acetone/butanol/ethanol (ABE) yield of 0.41 g g(-1). When non-detoxified SAHHC was used as carbon source, C. beijerinckii NCIMB 8052 grew well but ABE production was inhibited. By contrast, C. beijerinckii IB4 produced 9.5 g l(-1) of ABE with a yield of 0.34 g g(-1), including 2.2 g l(-1) acetone, 6.8 g l(-1) butanol, and 0.5 g l(-1) ethanol. The remarkable fermentation and inhibitor tolerance of C. beijerinckii IB4 appears promising for ABE production from lignocellulosic materials.
筛选出具有高抑制剂耐受性的凝结芽孢杆菌突变株 IB4，该菌株通过低能离子注入获得，并用于从经稀硫酸（SAHHC）处理的玉米纤维的未经解毒的半纤维素水解物中进行丁醇发酵。毒性评估表明，与出发菌株凝结芽孢杆菌 NCIMB 8052 相比，五种酚类化合物中有五种（例外是香草醛）的凝结芽孢杆菌 IB4 的耐受性更高。当使用葡萄糖作为碳源时，凝结芽孢杆菌 IB4 产生了 9.1 g/L 的丁醇，ABE 得率为 0.41 g/g。当使用未经解毒的 SAHHC 作为碳源时，凝结芽孢杆菌 NCIMB 8052 生长良好，但 ABE 生产受到抑制。相比之下，凝结芽孢杆菌 IB4 产生了 9.5 g/L 的 ABE，得率为 0.34 g/g，其中包括 2.2 g/L 的丙酮、6.8 g/L 的丁醇和 0.5 g/L 的乙醇。凝结芽孢杆菌 IB4 的显著发酵和抑制剂耐受性似乎有望用于从木质纤维素材料中生产 ABE。
